Overlapping regulation of CenH3 localization and histone H3 turnover by CAF-1 and HIR proteins in Saccharomyces cerevisiae.

July 18, 2021 Leave a comment
Correct chromosome segregation relies on the centromere-specific histone H3 isoform identified usually as CenH3, or as Cse4 in budding yeast. Cytological experiments have proven that Cse4 seems at extracentromeric loci in yeast cells poor for each the CAF-1 and HIR histone H3/H4 deposition complexes, per elevated nondisjunction in these double mutant cells. Right here, we examined molecular features of this Cse4 mislocalization. Genome-scale chromatin immunoprecipitation analyses demonstrated broader distribution of Cse4 exterior of centromeres in cac1Δ hir1Δ double mutant cells that lack each CAF-1 and HIR complexes than in both single mutant.
Nonetheless, cytological localization confirmed that the important inside kinetochore element Mif2 (CENP-C) was not recruited to extracentromeric Cse4 in cac1Δ hir1Δ double mutant cells. We additionally noticed that rpb1-1 mutants displayed a modestly elevated Cse4 half-life at nonpermissive temperatures, suggesting that turnover of Cse4 is partially depending on Pol II transcription. We used genome-scale assays to exhibit that the CAF-1 and HIR complexes independently stimulate replication-independent histone H3 turnover charges. We talk about methods through which altered histone change kinetics might have an effect on eviction of Cse4 from noncentromeric loci.

The leucine-rich repeat (LRR) protein, CaLRR1, interacts with the hypersensitive induced response (<em>HIR</em>) protein, Ca<em>HIR</em>1, and suppresses cell demise induced by the Ca<em>HIR</em>1 protein.

Leucine-rich repeat proteins (LRRs) operate in a lot of sign transduction pathways by way of protein-protein interactions. The gene encoding a small protein of pepper, CaLRR1, is particularly induced upon pathogen problem and therapy with pathogen-associated molecular patterns (PAMPs). We recognized a pepper hypersensitive induced response (CaHIR1) protein that interacts with the LRR area of the CaLRR1 protein utilizing yeast two-hybrid screening. Ectopic expression of the pepper CaHIR1 gene induces cell demise in tobacco and Arabidopsis, indicating that the CaHIR1 protein could also be a constructive regulator of HR-like cell demise.
Overlapping regulation of CenH3 localization and histone H3 turnover by CAF-1 and HIR proteins in Saccharomyces cerevisiae.
As a result of transformation may be very troublesome in pepper vegetation, we over-expressed CaLRR1 and CaHIR1 in Arabidopsis to find out mobile capabilities of the 2 genes. The over-expression of the CaHIR1 gene, however not the CaLRR1 gene, in transgenic Arabidopsis confers illness resistance in response to Pseudomonas syringae an infection, accompanied by the sturdy expression of PR genes, the buildup of each salicylic acid and H(2)O(2), and Okay(+) efflux in plant cells.
In Arabidopsis and tobacco vegetation over-expressing each CaHIR1 and CaLRR1, the CaLRR1 protein suppresses not solely CaHIR1-induced cell demise, but in addition PR gene expression elicited by CaHIR1 by way of its affiliation with HIR protein. We suggest that the CaLRR1 protein capabilities as a novel damaging regulator of CaHIR1-mediated cell demise responses in vegetation.

FIP2 and Rip11 specify Rab11a-mediated mobile distribution of GLUT4 and FAT/CD36 in H9c2-hIR cells.

Rab11a has been proven to be concerned in numerous vesicle trafficking processes. To additional outline the purposeful position of Rab11a in vesicle motion we knocked down gene expression of Rab11a and two of its effectors, Rip11 and FIP2, in H9c2-hIR cells and measured the cell floor abundance of GLUT4myc and FAT/CD36. We noticed that by flattening Rab11a, each GLUT4myc and FAT/CD36 abundance on the plasma membrane had been considerably elevated. Within the case of GLUT4myc, the in vitro knockdown of FIP2 additionally elevated the cell floor abundance of GLUT4myc.
Knockdown of each FIP2 and Rip11 improve the abundance of FAT/CD36 on the plasma membrane. Stimulated translocation of GLUT4myc and FAT/CD36 is just not altered after gene knockdown of Rab11a. These knowledge subsequently present that (i) Rab11a regulates cell floor abundance of each GLUT4 and FAT/CD36 and that (ii) each Rab11a-dependent processes are in another way regulated by Rab11a effector proteins.

Replication-independent histone deposition by the HIR advanced and Asf1.

The orderly deposition of histones onto DNA is mediated by conserved meeting complexes, together with chromatin meeting factor-1 (CAF-1) and the Hir proteins . CAF-1 and the Hir proteins function in distinct however functionally overlapping histone deposition pathways in vivo . The Hir proteins and CAF-1 share a typical associate, the extremely conserved histone H3/H4 binding protein Asf1, which binds the center subunit of CAF-1 in addition to to Hir proteins . Asf1 binds to newly synthesized histones H3/H4 , and this advanced stimulates histone deposition by CAF-1 .

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FNab03871 100µg
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Description: Antibody raised against HIRIP3

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E45R16639G-4 50 ul
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Description: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

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E-AB-61469-120uL 120uL
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Description: Unconjugated

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Description: Unconjugated

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Description: Unconjugated

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Description: Unconjugated

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Description: Unconjugated

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Description: Unconjugated

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EUR 143
Description: Unconjugated

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Description: Unconjugated

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Description: Unconjugated

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RD218848A-120uL 120μL
EUR 360
Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

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RD218848A-200uL 200μL
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Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

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Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

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RD218848A-60uL 60μL
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Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

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RD84223A-120uL 120μL
EUR 360
Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

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RD84223A-200uL 200μL
EUR 630
Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

HIRIP3 Polyclonal Antibody
RD84223A-60uL 60μL
EUR 204
Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

HIRIP3 Polyclonal Antibody
RD252761A-120uL 120μL
EUR 360
Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

HIRIP3 Polyclonal Antibody
RD252761A-200uL 200μL
EUR 630
Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

HIRIP3 Polyclonal Antibody
RD252761A-20uL 20μL
EUR 109.5
Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

HIRIP3 Polyclonal Antibody
RD252761A-60uL 60μL
EUR 214.5
Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

HIRIP3 antibody - middle region
MBS3213825-01mL 0.1mL
EUR 455

HIRIP3 antibody - middle region
MBS3213825-5x01mL 5x0.1mL
EUR 1995

OAAN03627-50UL - HIRIP3 Antibody
OAAN03627-50UL 50ul
EUR 149

Polyclonal HIRIP3 Antibody (Center)
APR16639G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HIRIP3 (Center). This antibody is tested and proven to work in the following applications:

OAAN03627-100UL - HIRIP3 Antibody
OAAN03627-100UL 100ul
EUR 249

OAAN03627-200UL - HIRIP3 Antibody
OAAN03627-200UL 200ul
EUR 389

HIRIP3 Rabbit Polyclonal Antibody
E10G02926 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

HIRIP3 Rabbit Polyclonal Antibody
E10G09929 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

HIRIP3 Rabbit Polyclonal Antibody
E10G32013 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

HIRIP3 Antibody (Center) Blocking Peptide
MBS9223588-INQUIRE INQUIRE Ask for price

HIRIP3 Polyclonal Conjugated Antibody
C31854 100ul
EUR 476.4

HIRIP3 Polyclonal Conjugated Antibody
C30792 100ul
EUR 476.4

HIRIP3 Polyclonal Conjugated Antibody
MBS9441968-01mLAF350 0.1mL(AF350)
EUR 480

HIRIP3 Polyclonal Conjugated Antibody
MBS9441968-01mLAF405 0.1mL(AF405)
EUR 480

HIRIP3 Polyclonal Conjugated Antibody
MBS9441968-01mLAF488 0.1mL(AF488)
EUR 480

HIRIP3 Polyclonal Conjugated Antibody
MBS9441968-01mLAF555 0.1mL(AF555)
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HIRIP3 Polyclonal Conjugated Antibody
MBS9441968-01mLBiotin 0.1mL(Biotin)
EUR 480

HIRIP3 Polyclonal Conjugated Antibody
MBS9443027-01mLAF350 0.1mL(AF350)
EUR 480

HIRIP3 Polyclonal Conjugated Antibody
MBS9443027-01mLAF405 0.1mL(AF405)
EUR 480

HIRIP3 Polyclonal Conjugated Antibody
MBS9443027-01mLAF488 0.1mL(AF488)
EUR 480

HIRIP3 Polyclonal Conjugated Antibody
MBS9443027-01mLAF555 0.1mL(AF555)
EUR 480

HIRIP3 Polyclonal Conjugated Antibody
MBS9443027-01mLBiotin 0.1mL(Biotin)
EUR 480

HIRA-Interacting Protein 3 (HIRIP3) Antibody
abx030421-400l 400 µl
EUR 518.75

HIRA-Interacting Protein 3 (HIRIP3) Antibody
abx030421-400ul 400 ul
EUR 627.6

HIRA-Interacting Protein 3 (HIRIP3) Antibody
abx030421-80l 80 µl
EUR 281.25

HIRA-Interacting Protein 3 (HIRIP3) Antibody
abx037953-100g 100 µg
EUR 337.5

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abx037953-100ug 100 ug
EUR 469.2

HIRA-Interacting Protein 3 (HIRIP3) Antibody
abx005315-100l 100 µl
EUR 400

HIRA-Interacting Protein 3 (HIRIP3) Antibody
20-abx005315
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  • 20 ul
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HIRA-Interacting Protein 3 (HIRIP3) Antibody
abx005315-20l 20 µl
EUR 175

HIRA-Interacting Protein 3 (HIRIP3) Antibody
abx005315-50l 50 µl
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HIRA-Interacting Protein 3 (HIRIP3) Antibody
abx113016-100l 100 µl
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HIRA-Interacting Protein 3 (HIRIP3) Antibody
20-abx113016
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  • 50 ul
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HIRA-Interacting Protein 3 (HIRIP3) Antibody
abx233871-100g 100 µg
EUR 350

HIRA-Interacting Protein 3 (HIRIP3) Antibody
abx233871-100ug 100 ug
EUR 661.2

ARP58286_P050-25UL - HIRIP3 Antibody - middle region
ARP58286_P050-25UL 25ul
EUR 99

HIRIP3
CSB-CL883612HU 10 μg plasmid + 200μl Glycerol Ask for price

HIRIP3
MBS8536180-01mL 0.1mL
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HIRIP3
MBS8536180-01mLAF405L 0.1mL(AF405L)
EUR 565

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MBS8536180-01mLAF405S 0.1mL(AF405S)
EUR 565

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MBS8536180-01mLAF610 0.1mL(AF610)
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HIRIP3
MBS8536180-01mLAF635 0.1mL(AF635)
EUR 565

ARP58286_P050 - HIRIP3 antibody - middle region (ARP58286_P050)
ARP58286_P050 100ul
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Mouse monoclonal antibody Anti-Human HIRIP3
MBS120315-01mg 0.1mg
EUR 470

Mouse monoclonal antibody Anti-Human HIRIP3
MBS120315-5x01mg 5x0.1mg
EUR 2080

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3)
MBS6002458-02mL 0.2(mL
EUR 695

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3)
MBS6002458-5x02mL 5x0.2mL
EUR 2975

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (AP)
MBS6306626-02mL 0.2mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (AP)
MBS6306626-5x02mL 5x0.2mL
EUR 4250

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (PE)
MBS6306636-02mL 0.2mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (PE)
MBS6306636-5x02mL 5x0.2mL
EUR 4250

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (APC)
MBS6306627-02mL 0.2mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (APC)
MBS6306627-5x02mL 5x0.2mL
EUR 4250

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (FITC)
MBS6306629-02mL 0.2mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (FITC)
MBS6306629-5x02mL 5x0.2mL
EUR 4250

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (Biotin)
MBS6306628-02mL 0.2mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (Biotin)
MBS6306628-5x02mL 5x0.2mL
EUR 4250

HIRIP3 (untagged)-Human HIRA interacting protein 3 (HIRIP3)
SC117881 10 µg Ask for price

HIRIP3 (untagged)-Human HIRA interacting protein 3 (HIRIP3)
SC324592 10 µg Ask for price

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (MaxLight 405)
MBS6306631-01mL 0.1mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (MaxLight 405)
MBS6306631-5x01mL 5x0.1mL
EUR 4250

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (MaxLight 490)
MBS6306632-01mL 0.1mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (MaxLight 490)
MBS6306632-5x01mL 5x0.1mL
EUR 4250

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (MaxLight 550)
MBS6306633-01mL 0.1mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (MaxLight 550)
MBS6306633-5x01mL 5x0.1mL
EUR 4250

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (MaxLight 650)
MBS6306634-01mL 0.1mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (MaxLight 650)
MBS6306634-5x01mL 5x0.1mL
EUR 4250

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (MaxLight 750)
MBS6306635-01mL 0.1mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (MaxLight 750)
MBS6306635-5x01mL 5x0.1mL
EUR 4250

Hirip3 (GFP-tagged) - Mouse HIRA interacting protein 3 (Hirip3)
MG209311 10 µg Ask for price

HIRIP3 (GFP-tagged) - Human HIRA interacting protein 3 (HIRIP3)
RG200510 10 µg Ask for price

Hirip3 (untagged) - Mouse HIRA interacting protein 3 (Hirip3), (10ug)
MC219487 10 µg Ask for price

HIRIP3 Rabbit pAb
A7014 50μL
EUR 262.15

HIRIP3 Rabbit pAb
A7014-200ul 200 ul
EUR 550.8

HIRIP3 Rabbit pAb
A7014-20ul 20 ul
EUR 219.6

HIRIP3 cDNA Clone
MBS1266829-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

HIRIP3 cDNA Clone
MBS1266829-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

HIRIP3 Rabbit pAb
E2507014 100ul
EUR 225
Description: Available in various conjugation types.

HIRIP3 Rabbit pAb
E45R27025N 50 ul
EUR 297.75

HIRIP3 Rabbit pAb
MBS8546513-01mL 0.1mL
EUR 305

HIRIP3 Rabbit pAb
MBS8546513-01mLAF405L 0.1mL(AF405L)
EUR 565

HIRIP3 Rabbit pAb
MBS8546513-01mLAF405S 0.1mL(AF405S)
EUR 565

HIRIP3 Rabbit pAb
MBS8546513-01mLAF610 0.1mL(AF610)
EUR 565

HIRIP3 Rabbit pAb
MBS8546513-01mLAF635 0.1mL(AF635)
EUR 565

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (Azide free) (HRP)
MBS6306630-02mL 0.2mL
EUR 980

HIRIP3, ID (HIRIP3, HIRA-interacting protein 3) (Azide free) (HRP)
MBS6306630-5x02mL 5x0.2mL
EUR 4250

Mouse HIRIP3 siRNA
20-abx919397
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

Human HIRIP3 siRNA
20-abx919398
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

HIRIP3 siRNA (Mouse)
MBS8220953-15nmol 15nmol
EUR 405

HIRIP3 siRNA (Mouse)
MBS8220953-30nmol 30nmol
EUR 565

HIRIP3 siRNA (Mouse)
MBS8220953-5x30nmol 5x30nmol
EUR 2450

HIRIP3 siRNA (Human)
MBS8230588-15nmol 15nmol
EUR 405

HIRIP3 siRNA (Human)
MBS8230588-30nmol 30nmol
EUR 565

HIRIP3 siRNA (Human)
MBS8230588-5x30nmol 5x30nmol
EUR 2450

Hirip3 (Myc-DDK-tagged) - Mouse HIRA interacting protein 3 (Hirip3)
MR209311 10 µg Ask for price

HIRIP3 (Myc-DDK-tagged)-Human HIRA interacting protein 3 (HIRIP3)
RC200510 10 µg Ask for price

HIRIP3 cloning plasmid
CSB-CL883612HU-10ug 10ug
EUR 451.2
Description: A cloning plasmid for the HIRIP3 gene.

Hirip3 (untagged ORF) - Rat HIRA interacting protein 3 (Hirip3), (10 ug)
RN200696 10 µg Ask for price

HIRIP3 Blocking Peptide
33R-8235 100 ug
EUR 119
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of HIRIP3 antibody, catalog no. 70R-2185
In yeast, Asf1 is required for the contribution of the Hir proteins to gene silencing . Right here, we exhibit that Hir1, Hir2, Hir3, and Hpc2 comprise the HIR advanced, which copurifies with the histone deposition protein Asf1. Collectively, the HIR advanced and Asf1 deposit histones onto DNA in a replication-independent method. Histone deposition by the HIR advanced and Asf1 is impaired by a mutation in Asf1 that inhibits HIR binding. These knowledge point out that the HIR advanced and Asf1 proteins operate collectively as a conserved eukaryotic pathway for histone alternative all through the cell cycle.

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