Inadequate tissue oxygen, or hypoxia, is a central concept in the pathophysiology of ischemic disorders and cancer. Hypoxia promotes extracellular matrix (ECM) remodeling, cellular metabolic adaptation, and cancer cell metastasis. To discover new pathways through which cells respond to hypoxia, we performed a large-scale forward genetic screen in Caenorhabditis elegans and identified a previously uncharacterized receptor tyrosine kinase named HIR-1.
Loss of function in hir-1 phenocopied the impaired ECM integrity associated with hypoxia or deficiency in the oxygen-dependent dual oxidase, heme peroxidases, or cuticular collagens involved in ECM homeostasis. Genetic suppressor screens identified NHR-49 and MDT-15 as transcriptional regulators downstream of HIR-1.
Furthermore, hir-1 mutants showed defects in adapting to and recovering from prolonged severe hypoxia. We propose that C. elegans HIR-1 coordinates hypoxia-inducible factor-independent responses to hypoxia and hypoxia-associated ECM remodeling through mechanisms that are likely conserved in other organisms.
The Candida albicans HIR histone chaperone regulates the yeast-to-hyphae transition by controlling the sensitivity to morphogenesis signals.
Morphological plasticity such as the yeast-to-hyphae transition is a key virulence factor of the human fungal pathogen Candida albicans. Hyphal formation is controlled by a multilayer regulatory network composed of environmental sensing, signaling, transcriptional modulators as well as chromatin modifications. Here, we demonstrate a novel role for the replication-independent HIR histone chaperone complex in fungal morphogenesis. HIR operates as a crucial modulator of hyphal development, since genetic ablation of the HIR complex subunit Hir1 decreases sensitivity to morphogenetic stimuli.
Strikingly, HIR1-deficient cells display altered transcriptional amplitudes upon hyphal initiation, suggesting that Hir1 affects transcription by establishing transcriptional thresholds required for driving morphogenetic cell-fate decisions. Furthermore, ectopic expression of the transcription factor Ume6, which facilitates hyphal maintenance, rescues filamentation defects of hir1Δ/Δ cells, suggesting that Hir1 impacts the early phase of hyphal initiation. Hence, chromatin chaperone-mediated fine-tuning of transcription is crucial for driving morphogenetic conversions in the fungal pathogen C. albicans.
The CAF-1 and Hir Histone Chaperones Associate with Sites of Meiotic Double-Strand Breaks in Budding Yeast.
In the meiotic prophase, programmed DNA double-strand breaks (DSB) are introduced along chromosomes to promote homolog pairing and recombination. Although meiotic DSBs usually occur in nucleosome-depleted, accessible regions of chromatin, their repair by homologous recombination takes place in a nucleosomal environment. Nucleosomes may represent an obstacle for the recombination machinery and their timely eviction and reincorporation into chromatin may influence the outcome of recombination, for instance by stabilizing recombination intermediates.
Here we show in budding yeast that nucleosomes flanking a meiotic DSB are transiently lost during recombination, and that specific histone H3 chaperones, CAF-1 and Hir, are mobilized at meiotic DSBs. However, the absence of these chaperones has no effect on meiotic recombination, suggesting that timely histone reincorporation following their eviction has no influence on the recombination outcome, or that redundant pathways are activated. This study is the first example of the involvement of histone H3 chaperones at naturally occurring, developmentally programmed DNA double-strand breaks.
The histone chaperone complex HIR maintains nucleosome occupancy and counterbalances impaired histone deposition in CAF-1 complex mutants.
Chromatin organization is essential for coordinated gene expression, genome stability, and inheritance of epigenetic information. The main components involved in chromatin assembly are specific complexes such as Chromatin Assembly Factor 1 (CAF-1) and Histone Regulator (HIR), which deposit histones in a DNA synthesis-dependent or -independent manner, respectively. Here, we characterize the role of the plant orthologs Histone Regulator A (HIRA), Ubinuclein (UBN) and Calcineurin Binding protein 1 (CABIN1), which constitute the HIR complex.
Arabidopsis loss-of-function mutants for the various subunits of the complex are viable, but hira mutants show reduced fertility. We show that loss of HIRA reduces extractable histone H3 protein levels and decreases nucleosome occupancy at both actively transcribed genes and heterochromatic regions. Concomitantly, HIRA contributes to maintenance of silencing of pericentromeric repeats and certain transposons.
A genetic analysis based on crosses between mutants deficient in subunits of the CAF-1 and HIR complexes showed that simultaneous loss of both the CAF-1 and HIR histone H3 chaperone complexes severely affects plant survival, growth and reproductive development. Our results suggest that HIRA partially rescues impaired histone deposition in fas mutants to preserve nucleosome occupancy, implying plasticity in histone variant interaction and deposition.
Tissue-specific expression of a soybean hypersensitive-induced response (HIR) protein gene promoter.
A Glycine max gene encoding a putative protein similar to hypersensitive-induced response proteins (HIR) was identified as a gene with preferred expressions in flowers and developing seeds by whole transcriptome gene expression profiling. Its promoter gm-hir1 was cloned and revealed to strongly express a fluorescence reporter gene primarily in integuments, anther tapetum, and seed coat with unique tissue-specificity. Expression in the inner integument was apparent prior to pollination, while expression in the outer integument started to develop from the micropylar end outward as the embryo matured.
Phospho-DRP1 (Ser616) Antibody |
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| DF2972 | Affbiotech | 200ul | EUR 420 |
Kv4.2/KCND2 (Phospho-Ser616) Antibody |
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| 12447-100ul | SAB | 100ul | EUR 302.4 |
Kv4.2/KCND2 (Phospho-Ser616) Antibody |
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| 12447-50ul | SAB | 50ul | EUR 224.4 |
Kv4.2 (phospho Ser616) Polyclonal Antibody |
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| ABP54999-003ml | Abbkine | 0.03ml | EUR 189.6 |
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Description: A polyclonal antibody for detection of Kv4.2 phospho Ser616) from Human, Mouse, Rat. This Kv4.2 phospho Ser616) antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Kv4.2 around the phosphorylation site of S616 |
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Kv4.2 (phospho Ser616) Polyclonal Antibody |
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| ABP54999-01ml | Abbkine | 0.1ml | EUR 346.8 |
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Description: A polyclonal antibody for detection of Kv4.2 phospho Ser616) from Human, Mouse, Rat. This Kv4.2 phospho Ser616) antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Kv4.2 around the phosphorylation site of S616 |
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Kv4.2 (phospho Ser616) Polyclonal Antibody |
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| ABP54999-02ml | Abbkine | 0.2ml | EUR 496.8 |
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Description: A polyclonal antibody for detection of Kv4.2 phospho Ser616) from Human, Mouse, Rat. This Kv4.2 phospho Ser616) antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human Kv4.2 around the phosphorylation site of S616 |
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Kv4.2/KCND2 (Phospho- Ser616) Antibody |
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| ABF8074 | Lifescience Market | 100 ug | EUR 525.6 |
Phospho-Kv4.2/KCND2 (Ser616) Antibody |
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| AF8074 | Affbiotech | 200ul | EUR 540 |
Kv4.2 (phospho Ser616) Polyclonal Antibody |
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| ES5998-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against Kv4.2 (phospho Ser616) from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA |
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Kv4.2 (phospho Ser616) Polyclonal Antibody |
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| ES5998-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against Kv4.2 (phospho Ser616) from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA |
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IRS-1 (phospho Ser616) Polyclonal Antibody |
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| ABP54928-003ml | Abbkine | 0.03ml | EUR 189.6 |
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Description: A polyclonal antibody for detection of IRS-1 phospho Ser616) from Human, Mouse, Rat. This IRS-1 phospho Ser616) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human IRS-1 around the phosphorylation site of S616 |
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IRS-1 (phospho Ser616) Polyclonal Antibody |
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| ABP54928-01ml | Abbkine | 0.1ml | EUR 346.8 |
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Description: A polyclonal antibody for detection of IRS-1 phospho Ser616) from Human, Mouse, Rat. This IRS-1 phospho Ser616) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human IRS-1 around the phosphorylation site of S616 |
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IRS-1 (phospho Ser616) Polyclonal Antibody |
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| ABP54928-02ml | Abbkine | 0.2ml | EUR 496.8 |
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Description: A polyclonal antibody for detection of IRS-1 phospho Ser616) from Human, Mouse, Rat. This IRS-1 phospho Ser616) antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human IRS-1 around the phosphorylation site of S616 |
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DRP1 (Phospho-Ser616) Polyclonal Conjugated Antibody |
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| C12749 | SAB | 100ul | EUR 476.4 |
IRS-1 (phospho Ser616) Polyclonal Antibody |
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| ES5927-100ul | ELK Biotech | 100ul | EUR 334.8 |
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Description: A Rabbit Polyclonal antibody against IRS-1 (phospho Ser616) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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IRS-1 (phospho Ser616) Polyclonal Antibody |
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| ES5927-50ul | ELK Biotech | 50ul | EUR 248.4 |
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Description: A Rabbit Polyclonal antibody against IRS-1 (phospho Ser616) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA |
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Phospho-IRS1 (S1101) Antibody |
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| 1-CSB-PA000553 | Cusabio |
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Description: A polyclonal antibody against Phospho-IRS1 (S1101). Recognizes Phospho-IRS1 (S1101) from Human, Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
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Phospho-IRS1 (S307) Antibody |
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| 1-CSB-PA000554 | Cusabio |
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Description: A polyclonal antibody against Phospho-IRS1 (S307). Recognizes Phospho-IRS1 (S307) from Human, Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000 |
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Phospho-IRS1 (S307) Antibody |
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| 1-CSB-PA000555 | Cusabio |
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Description: A polyclonal antibody against Phospho-IRS1 (S307). Recognizes Phospho-IRS1 (S307) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/10000 |
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Phospho-IRS1 (S636) Antibody |
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| 1-CSB-PA000556 | Cusabio |
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Description: A polyclonal antibody against Phospho-IRS1 (S636). Recognizes Phospho-IRS1 (S636) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000 |
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Phospho-IRS1 (S639) Antibody |
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| 1-CSB-PA000557 | Cusabio |
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Description: A polyclonal antibody against Phospho-IRS1 (S639). Recognizes Phospho-IRS1 (S639) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
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Phospho-IRS1 (S794) Antibody |
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| 1-CSB-PA000558 | Cusabio |
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Description: A polyclonal antibody against Phospho-IRS1 (S794). Recognizes Phospho-IRS1 (S794) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/40000 |
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Phospho-IRS1 (S312) Antibody |
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| 1-CSB-PA000667 | Cusabio |
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Description: A polyclonal antibody against Phospho-IRS1 (S312). Recognizes Phospho-IRS1 (S312) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/20000 |
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Phospho-IRS1 (Ser636) Antibody |
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| CSB-PA577877-100ul | Cusabio | 100ul | EUR 434.4 |
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Description: A polyclonal antibody against Phospho-IRS1 (Ser636). Recognizes Phospho-IRS1 (Ser636) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100 |
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Phospho-IRS1 (Ser307) Antibody |
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| AF3272 | Affbiotech | 200ul | EUR 420 |
Phospho-IRS1 (Ser312) Antibody |
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| AF3273 | Affbiotech | 200ul | EUR 420 |
Phospho-IRS1 (Ser636) Antibody |
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| AF3274 | Affbiotech | 200ul | EUR 420 |
Phospho-IRS1 (Ser639) Antibody |
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| AF3275 | Affbiotech | 200ul | EUR 420 |
Phospho-IRS1 (Tyr612) Antibody |
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| AF3677 | Affbiotech | 100ul | EUR 540 |
Phospho-IRS1 (Tyr989) Antibody |
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| AF3807 | Affbiotech | 100ul | EUR 540 |
Phospho-IRS1 (Ser1101) Antibody |
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| AF4421 | Affbiotech | 200ul | EUR 540 |
Phospho-IRS1 (Tyr896) Antibody |
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| AF4424 | Affbiotech | 200ul | EUR 540 |
Phospho-IRS1 (Ser312) Antibody |
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| CSB-PA174165-100ul | Cusabio | 100ul | EUR 434.4 |
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Description: A polyclonal antibody against Phospho-IRS1 (Ser312). Recognizes Phospho-IRS1 (Ser312) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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Phospho-IRS1 (Ser307) Antibody |
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| CSB-PA224848-100ul | Cusabio | 100ul | EUR 434.4 |
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Description: A polyclonal antibody against Phospho-IRS1 (Ser307). Recognizes Phospho-IRS1 (Ser307) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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Phospho-IRS1 (Ser639) Antibody |
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| CSB-PA004394-100ul | Cusabio | 100ul | EUR 434.4 |
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Description: A polyclonal antibody against Phospho-IRS1 (Ser639). Recognizes Phospho-IRS1 (Ser639) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100 |
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Phospho-IRS1 (Y896) Antibody |
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| 1-CSB-PA011243 | Cusabio |
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Description: A polyclonal antibody against Phospho-IRS1 (Y896). Recognizes Phospho-IRS1 (Y896) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/40000 |
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Phospho-IRS1 (S616) Antibody |
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| 1-CSB-PA011244 | Cusabio |
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Description: A polyclonal antibody against Phospho-IRS1 (S616). Recognizes Phospho-IRS1 (S616) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000 |
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Phospho-IRS1 (S323) Antibody |
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| 1-CSB-PA011245 | Cusabio |
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Description: A polyclonal antibody against Phospho-IRS1 (S323). Recognizes Phospho-IRS1 (S323) from Human, Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/10000 |
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Phospho-IRS1 (Ser639) Antibody |
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| CSB-PA004394- | Cusabio | each | EUR 402 |
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Description: A polyclonal antibody against Phospho-IRS1 (Ser639). Recognizes Phospho-IRS1 (Ser639) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100 |
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Phospho-IRS1 (Ser312) Antibody |
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| CSB-PA174165- | Cusabio | each | EUR 402 |
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Description: A polyclonal antibody against Phospho-IRS1 (Ser312). Recognizes Phospho-IRS1 (Ser312) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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Phospho-IRS1 (Ser307) Antibody |
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| CSB-PA224848- | Cusabio | each | EUR 402 |
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Description: A polyclonal antibody against Phospho-IRS1 (Ser307). Recognizes Phospho-IRS1 (Ser307) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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Phospho-IRS1 (Ser636) Antibody |
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| CSB-PA577877- | Cusabio | each | EUR 402 |
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Description: A polyclonal antibody against Phospho-IRS1 (Ser636). Recognizes Phospho-IRS1 (Ser636) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100 |
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Phospho-DRP1 (Ser616) Blocking Peptide |
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| AF8470-BP | Affbiotech | 1mg | EUR 234 |
Phospho-DRP1 (Ser616) Blocking Peptide |
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| DF2972-BP | Affbiotech | 1mg | EUR 234 |
Kv4.2/KCND2 (Phospho-Ser616) Polyclonal Conjugated Antibody |
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| C12447 | SAB | 100ul | EUR 476.4 |
Phospho-IRS (Ser616) Antibody (Clone HIR-B1) |
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| 3105-100 | Biovision | each | EUR 430.8 |
Phospho-IRS1 (Ser318)[Ser323] Antibody |
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| AF4422 | Affbiotech | 200ul | EUR 540 |
Anti-Phospho-IRS1-S1101 antibody |
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| STJ11101007 | St John's Laboratory | 100 µl | EUR 471.6 |
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Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. |
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Anti-Phospho-IRS1-S323 antibody |
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| STJ11101089 | St John's Laboratory | 100 µl | EUR 471.6 |
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Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. |
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Anti-Phospho-IRS1-(S307) antibody |
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| STJ110879 | St John's Laboratory | 100 µl | EUR 471.6 |
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Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. |
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Anti-Phospho-IRS1-(S312) antibody |
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| STJ22196 | St John's Laboratory | 100 µl | EUR 471.6 |
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Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. |
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Anti-Phospho-IRS1-(S636) antibody |
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| STJ22197 | St John's Laboratory | 100 µl | EUR 471.6 |
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Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. |
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Anti-Phospho-IRS1-(S639) antibody |
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| STJ22198 | St John's Laboratory | 100 µl | EUR 471.6 |
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Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. |
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Anti-Phospho-IRS1-(S307) antibody |
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| STJ22199 | St John's Laboratory | 100 µl | EUR 471.6 |
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Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. |
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Phospho-Kv4.2/KCND2 (Ser616) Blocking Peptide |
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| AF8074-BP | Affbiotech | 1mg | EUR 234 |
Phospho-IRS-1 (Ser616) Blocking Peptide |
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| AF4423-BP | Affbiotech | 1mg | EUR 234 |
KCND2 antibody (Ser616) |
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| 70R-35925 | Fitzgerald | 100 ug | EUR 392.4 |
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Description: Rabbit polyclonal KCND2 antibody (Ser616) |
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anti-IRS1 (Phospho-Ser612) |
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| LF-PA20649 | Abfrontier | 100 ul | EUR 424.8 |
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Description: Rabbit polyclonal to IRS1 (Phospho-Ser612) |
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Dynamin-1-Like Protein Phospho-Ser616 (DRP1) Antibody |
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| 20-abx149897 | Abbexa |
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Anti-Phospho-IRS1-S636/639 antibody |
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| STJ11101023 | St John's Laboratory | 100 µl | EUR 471.6 |
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Description: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. |
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Human Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| DLR-IRS1-Hu-48T | DL Develop | 48T | EUR 620.4 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Insulin Receptor Substrate 1 (IRS1) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Human Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| DLR-IRS1-Hu-96T | DL Develop | 96T | EUR 807.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Insulin Receptor Substrate 1 (IRS1) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Mouse Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| DLR-IRS1-Mu-48T | DL Develop | 48T | EUR 632.4 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Insulin Receptor Substrate 1 (IRS1) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Mouse Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| DLR-IRS1-Mu-96T | DL Develop | 96T | EUR 825.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Insulin Receptor Substrate 1 (IRS1) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Rat Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| DLR-IRS1-Ra-48T | DL Develop | 48T | EUR 658.8 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Insulin Receptor Substrate 1 (IRS1) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Rat Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| DLR-IRS1-Ra-96T | DL Develop | 96T | EUR 861.6 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Insulin Receptor Substrate 1 (IRS1) in samples from tissue homogenates, cell lysates or other biological fluids. |
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Human Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RDR-IRS1-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 652.8 |
Human Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RDR-IRS1-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 907.2 |
Mouse Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RDR-IRS1-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 668.4 |
Mouse Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RDR-IRS1-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 928.8 |
Rat Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RDR-IRS1-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 699.6 |
Rat Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RDR-IRS1-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 973.2 |
Human Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RD-IRS1-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 625.2 |
Human Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RD-IRS1-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 867.6 |
Mouse Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RD-IRS1-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 639.6 |
Mouse Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RD-IRS1-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 888 |
Rat Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RD-IRS1-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 668.4 |
Rat Insulin Receptor Substrate 1 (IRS1) ELISA Kit |
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| RD-IRS1-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 930 |
Rat Phospho-IRS1 (Ser307) (p-IRS1 Ser307) ELISA kit |
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| YLA1466RA-48T | Shanghai YL Biotech | 48T | EUR 465 |
Rat Phospho-IRS1 (Ser307) (p-IRS1 Ser307) ELISA kit |
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| YLA1466RA-96T | Shanghai YL Biotech | 96T | EUR 600 |
Phospho-IRS1-S312 Rabbit pAb |
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| AP0181-100ul | Abclonal | 100 ul | EUR 460.8 |
Phospho-IRS1-S312 Rabbit pAb |
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| AP0181-200ul | Abclonal | 200 ul | EUR 664.8 |
Phospho-IRS1-S312 Rabbit pAb |
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| AP0181-20ul | Abclonal | 20 ul | Ask for price |
Phospho-IRS1-S312 Rabbit pAb |
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| AP0181-50ul | Abclonal | 50 ul | EUR 318 |
Phospho-IRS1-S636 Rabbit pAb |
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| AP0225-100ul | Abclonal | 100 ul | EUR 460.8 |
Phospho-IRS1-S636 Rabbit pAb |
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| AP0225-200ul | Abclonal | 200 ul | EUR 664.8 |
Phospho-IRS1-S636 Rabbit pAb |
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| AP0225-20ul | Abclonal | 20 ul | Ask for price |
Phospho-IRS1-S636 Rabbit pAb |
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| AP0225-50ul | Abclonal | 50 ul | EUR 318 |
Phospho-IRS1-S639 Rabbit pAb |
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| AP0226-100ul | Abclonal | 100 ul | EUR 460.8 |
Phospho-IRS1-S639 Rabbit pAb |
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| AP0226-200ul | Abclonal | 200 ul | EUR 664.8 |
Phospho-IRS1-S639 Rabbit pAb |
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| AP0226-20ul | Abclonal | 20 ul | Ask for price |
Phospho-IRS1-S639 Rabbit pAb |
|||
| AP0226-50ul | Abclonal | 50 ul | EUR 318 |
Phospho-IRS1-S307 Rabbit pAb |
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| AP0371-100ul | Abclonal | 100 ul | EUR 460.8 |
Phospho-IRS1-S307 Rabbit pAb |
|||
| AP0371-200ul | Abclonal | 200 ul | EUR 664.8 |
Phospho-IRS1-S307 Rabbit pAb |
|||
| AP0371-20ul | Abclonal | 20 ul | Ask for price |
Phospho-IRS1-S307 Rabbit pAb |
|||
| AP0371-50ul | Abclonal | 50 ul | EUR 318 |
Phospho-IRS1-S307 Rabbit pAb |
|||
| AP0552-100ul | Abclonal | 100 ul | EUR 460.8 |
Phospho-IRS1-S307 Rabbit pAb |
|||
| AP0552-200ul | Abclonal | 200 ul | EUR 664.8 |
Phospho-IRS1-S307 Rabbit pAb |
|||
| AP0552-20ul | Abclonal | 20 ul | EUR 219.6 |
Phospho-IRS1-S307 Rabbit pAb |
|||
| AP0552-50ul | Abclonal | 50 ul | EUR 318 |
Phospho-IRS1-S1101 Rabbit pAb |
|||
| AP0867-100ul | Abclonal | 100 ul | EUR 460.8 |
Phospho-IRS1-S1101 Rabbit pAb |
|||
| AP0867-200ul | Abclonal | 200 ul | EUR 664.8 |
Phospho-IRS1-S1101 Rabbit pAb |
|||
| AP0867-20ul | Abclonal | 20 ul | EUR 219.6 |
Phospho-IRS1-S1101 Rabbit pAb |
|||
| AP0867-50ul | Abclonal | 50 ul | EUR 318 |
Phospho-IRS1-S323 Rabbit pAb |
|||
| AP0966-100ul | Abclonal | 100 ul | EUR 460.8 |
Phospho-IRS1-S323 Rabbit pAb |
|||
| AP0966-200ul | Abclonal | 200 ul | EUR 664.8 |
Phospho-IRS1-S323 Rabbit pAb |
|||
| AP0966-20ul | Abclonal | 20 ul | EUR 219.6 |
Phospho-IRS1-S323 Rabbit pAb |
|||
| AP0966-50ul | Abclonal | 50 ul | EUR 318 |
IRS1 antibody |
|||
| 70R-11816 | Fitzgerald | 100 ug | EUR 483.6 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
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| 70R-37602 | Fitzgerald | 100 ug | EUR 327.6 |
|
Description: Rabbit Polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-49927 | Fitzgerald | 100 ul | EUR 344.4 |
|
Description: Purified Polyclonal IRS1 antibody |
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IRS1 antibody |
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| 70R-49928 | Fitzgerald | 100 ul | EUR 344.4 |
|
Description: Purified Polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-49929 | Fitzgerald | 100 ul | EUR 292.8 |
|
Description: Purified Polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-34209 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-34211 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-34213 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-34215 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-35306 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Purified Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-36824 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit Polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-18013 | Fitzgerald | 50 ul | EUR 522 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-31319 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 70R-31321 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 38112-100ul | SAB | 100ul | EUR 302.4 |
IRS1 Antibody |
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| 49156-100ul | SAB | 100ul | EUR 399.6 |
IRS1 Antibody |
|||
| 49156-50ul | SAB | 50ul | EUR 286.8 |
IRS1 Antibody |
|||
| 35792-100ul | SAB | 100ul | EUR 302.4 |
IRS1 antibody |
|||
| 20R-1996 | Fitzgerald | 50 ug | EUR 337.2 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 20R-2070 | Fitzgerald | 50 ug | EUR 337.2 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 20R-2071 | Fitzgerald | 50 ug | EUR 337.2 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 20R-2386 | Fitzgerald | 50 ug | EUR 337.2 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 antibody |
|||
| 20R-2387 | Fitzgerald | 50 ug | EUR 337.2 |
|
Description: Rabbit polyclonal IRS1 antibody |
|||
IRS1 Antibody |
|||
| ABD6048 | Lifescience Market | 100 ug | EUR 525.6 |
IRS1 Antibody |
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| AF6807 | Affbiotech | 100ul | EUR 420 |
IRS1 Antibody |
|||
| DF6048 | Affbiotech | 200ul | EUR 420 |
IRS1 Antibody |
|||
| AF4721 | Affbiotech | 200ul | EUR 540 |
IRS1 Antibody |
|||
| AF4722 | Affbiotech | 200ul | EUR 540 |
IRS1 Antibody |
|||
| AF4723 | Affbiotech | 200ul | EUR 540 |
IRS1 Antibody |
|||
| AF4724 | Affbiotech | 200ul | EUR 540 |
IRS1 Antibody |
|||
| AF6272 | Affbiotech | 200ul | EUR 420 |
IRS1 Antibody |
|||
| AF6273 | Affbiotech | 200ul | EUR 420 |
IRS1 Antibody |
|||
| AF6677 | Affbiotech | 100ul | EUR 420 |
IRS1 Antibody |
|||
| 1-CSB-PA218932 | Cusabio |
|
|
|
Description: A polyclonal antibody against IRS1. Recognizes IRS1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200 |
|||
IRS1 Antibody |
|||
| 1-CSB-PA230288 | Cusabio |
|
|
|
Description: A polyclonal antibody against IRS1. Recognizes IRS1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100 |
|||
IRS1 Antibody |
|||
| 1-CSB-PA011247 | Cusabio |
|
|
|
Description: A polyclonal antibody against IRS1. Recognizes IRS1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/5000 |
|||
IRS1 Antibody |
|||
| 1-CSB-PA011248 | Cusabio |
|
|
|
Description: A polyclonal antibody against IRS1. Recognizes IRS1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/5000 |
|||
IRS1 Antibody |
|||
| 1-CSB-PA011249 | Cusabio |
|
|
|
Description: A polyclonal antibody against IRS1. Recognizes IRS1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000 |
|||
IRS1 Antibody |
|||
| 1-CSB-PA011250 | Cusabio |
|
|
|
Description: A polyclonal antibody against IRS1. Recognizes IRS1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000 |
|||
IRS1 Antibody |
|||
| 1-CSB-PA011829DSR1HU | Cusabio |
|
|
|
Description: A polyclonal antibody against IRS1. Recognizes IRS1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
|||
IRS1 Antibody |
|||
| 1-CSB-PA011829GA01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against IRS1. Recognizes IRS1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
|||
IRS1 Antibody |
|||
| 1-CSB-PA003065 | Cusabio |
|
|
|
Description: A polyclonal antibody against IRS1. Recognizes IRS1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000 |
|||
IRS1 Antibody |
|||
| 1-CSB-PA003068 | Cusabio |
|
|
|
Description: A polyclonal antibody against IRS1. Recognizes IRS1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000 |
|||
IRS1 Antibody |
|||
| R31636 | NSJ Bioreagents | 100 ug | EUR 419 |
IRS1 Antibody |
|||
| RQ5507 | NSJ Bioreagents | 100 ug | EUR 419 |
Phospho-IRS1-S636/639 Rabbit pAb |
|||
| AP0885-100ul | Abclonal | 100 ul | EUR 460.8 |
Phospho-IRS1-S636/639 Rabbit pAb |
|||
| AP0885-200ul | Abclonal | 200 ul | EUR 664.8 |
Phospho-IRS1-S636/639 Rabbit pAb |
|||
| AP0885-20ul | Abclonal | 20 ul | EUR 219.6 |
Phospho-IRS1-S636/639 Rabbit pAb |
|||
| AP0885-50ul | Abclonal | 50 ul | EUR 318 |
Rabbit Anti-Human IRS1 Polyclonal Antibody, Phospho-Ser312 |
|||
| CPB-1258RH | Creative Diagnostics | 100 ul | EUR 670.8 |
Rabbit Anti-Human IRS1 Polyclonal Antibody, Phospho-Ser636 |
|||
| CPB-768RH | Creative Diagnostics | 100 ul | EUR 670.8 |
Rabbit Anti-Human IRS1 Polyclonal Antibody, Phospho-Ser639 |
|||
| CPB-769RH | Creative Diagnostics | 100 ul | EUR 670.8 |
Monoclonal Phospho-IRS (Ser616) Antibody (Clone HIR-B1), Clone: HIR-B1 |
|||
| APR09169G | Leading Biology | 0.1mg | EUR 580.8 |
|
Description: A Monoclonal antibody against Human Phospho-IRS (Ser616) (Clone HIR-B1). The antibodies are raised in Mouse and are from clone HIR-B1. This antibody is applicable in WB, E |
|||
IRS1 antibody (Ser636) |
|||
| 70R-37296 | Fitzgerald | 100 ug | EUR 418.8 |
|
Description: Rabbit Polyclonal IRS1 antibody (Ser636) |
|||
IRS1 antibody (Ser639) |
|||
| 70R-37297 | Fitzgerald | 100 ug | EUR 418.8 |
|
Description: Rabbit Polyclonal IRS1 antibody (Ser639) |
|||
IRS1 antibody (Ser307) |
|||
| 70R-37301 | Fitzgerald | 100 ug | EUR 418.8 |
|
Description: Rabbit Polyclonal IRS1 antibody (Ser307) |
|||
IRS1 antibody (Ser612) |
|||
| 70R-37439 | Fitzgerald | 100 ug | EUR 418.8 |
|
Description: Rabbit Polyclonal IRS1 antibody (Ser612) |
|||
IRS1 antibody (Ser307) |
|||
| 70R-34208 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody (Ser307) |
|||
IRS1 antibody (Ser312) |
|||
| 70R-34210 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody (Ser312) |
|||
IRS1 antibody (Ser636) |
|||
| 70R-34212 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody (Ser636) |
|||
IRS1 antibody (Ser639) |
|||
| 70R-34214 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody (Ser639) |
|||
IRS1 antibody (Ser794) |
|||
| 70R-35835 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody (Ser794) |
|||
IRS1 antibody (Ser323) |
|||
| 70R-35928 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody (Ser323) |
|||
IRS1 antibody (Ser312) |
|||
| 70R-37221 | Fitzgerald | 100 ug | EUR 418.8 |
|
Description: Rabbit Polyclonal IRS1 antibody (Ser312) |
|||
IRS1 antibody (Ser307) |
|||
| 70R-31072 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody (Ser307) |
|||
IRS1 antibody (Ser1101) |
|||
| 70R-31318 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody (Ser1101) |
|||
IRS1 antibody (Ser612) |
|||
| 70R-31320 | Fitzgerald | 100 ug | EUR 392.4 |
|
Description: Rabbit polyclonal IRS1 antibody (Ser612) |
|||
IRS1 (pS307) Antibody |
|||
| 20-abx133119 | Abbexa |
|
|
A 5′-deletion study showed that the promoter can be truncated to 600 bp long relative to the translation start site without affecting expression. A positive regulatory element was identified between 600 and 481 bp that controls expression in the inner integument, with no noticeable effect on expression in the outer integument or tapetum. Additionally, removal of the 5’UTR intron had no effect on levels or location of gm-hir1 expression while truncation to 370 bp resulted in a complete loss of expression suggesting that elements controlling both the outer integument and tapetum expression are located within the 481-370 bp region.