RNA polymerase II initiates from low complexity sequences so cells must reliably distinguish “real” from “cryptic” promoters and maintain fidelity to the former. Further, this must be performed under a range of conditions, including those found within inactive and highly transcribed regions. Here, we used genome-scale screening to identify those factors that regulate the use of a specific cryptic promoter and how this is influenced by the degree of transcription over the element.
We show that promoter fidelity is most reliant on histone gene transactivators (Spt10, Spt21) and H3-H4 chaperones (Asf1, HIR complex) from the replication-independent deposition pathway. Mutations of Rtt106 that abrogate its interactions with H3-H4 or dsDNA permit extensive cryptic transcription comparable with replication-independent deposition factor deletions. We propose that nucleosome shielding is the primary means to maintain promoter fidelity, and histone replacement is most efficiently mediated in yeast cells by a HIR/Asf1/H3-H4/Rtt106 pathway.
Physical association of Arabidopsis hypersensitive induced reaction proteins (HIRs) with the immune receptor RPS2.
Arabidopsis RPS2 is a typical nucleotide-binding leucine-rich repeat resistance protein, which indirectly recognizes the bacterial effector protein AvrRpt2 and thereby activates effector-triggered immunity (ETI). Previously, we identified two hypersensitive induced reaction (AtHIR) proteins, AtHIR1 (At1g09840) and AtHIR2 (At3g01290), as potential RPS2 complex components.
AtHIR proteins contain the stomatin/prohibitin/flotillin/HflK/C domain (also known as the prohibitin domain or band 7 domain). In this study, we confirmed that AtHIR1 and AtHIR2 form complexes with RPS2 in Arabidopsis and Nicotiana benthamiana using a pulldown assay and fluorescence resonance energy transfer (FRET) analysis.
Arabidopsis has four HIR family genes (AtHIR1-4). All AtHIR proteins could form homo- and hetero-oligomers in vivo and were enriched in membrane microdomains of the plasma membrane. The mRNA levels of all except AtHIR4 were significantly induced by microbe-associated molecular patterns, such as the bacterial flagellin fragment flg22.
Athir2-1 and Athir3-1 mutants allowed more growth of Pto DC3000 AvrRpt2, but not Pto DC3000, indicating that these mutations reduce RPS2-mediated ETI but do not affect basal resistance to the virulent strain. Overexpression of AtHIR1 and AtHIR2 reduced growth of Pto DC3000. Taken together, the results show that the AtHIR proteins are physically associated with RPS2, are localized in membrane microdomains, and quantitatively contribute to RPS2-mediated ETI.
Separation-of-function mutation in HPC2, a member of the HIR complex in S. cerevisiae, results in derepression of the histone genes but does not confer cryptic TATA phenotypes.
The HIR complex, which is comprised of the four proteins Hir1, Hir2, Hir3 and Hpc2, was first characterized as a repressor of three of the four histone gene loci in Saccharomyces cerevisiae. Using a bioinformatical approach, previous studies have identified a region of Hpc2 that is conserved in Schizosaccharomyces pombe and humans. Using a similar approach, we identified two additional domains, CDI and CDII, of the Hpc2 protein that are conserved among yeast species related to S. cerevisiae.
We showed that the N terminal CDI domain (spanning amino acids 63-79) is dispensable for HIR complex assembly, but plays an essential role in the repression of the histone genes by recruiting the HIR complex to the HIR-dependent histone gene loci. The second conserved domain, CDII (spanning amino acids 452-480), is required for the stability of the Hpc2 protein itself as well as for the assembly of the HIR complex.
In addition, we report a novel separation-of-function mutation within CDI of Hpc2, which causes derepression of the histone genes but does not confer other reported hir/hpc- phenotypes (such as Spt phenotypes, heterochromatin silencing defects and repression of cryptic promoters). This is the first direct demonstration that a separation-of-function mutation exists within the HIR complex.
The Saccharomyces cerevisiae histone chaperone Rtt106 mediates the cell cycle recruitment of SWI/SNF and RSC to the HIR-dependent histone genes.
In Saccharomyces cerevisiae, three out of the four histone gene pairs (HTA1-HTB1, HHT1-HHF1, and HHT2-HHF2) are regulated by the HIR co-repressor complex. The histone chaperone Rtt106 has recently been shown to be present at these histone gene loci throughout the cell cycle in a HIR- and Asf1-dependent manner and involved in their transcriptional repression.
HIRIP3 Polyclonal Antibody |
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| 30792-50ul | SAB | 50ul | EUR 224.4 |
Polyclonal HIRIP3 Antibody |
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| APR02968G | Leading Biology | 0.05ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HIRIP3 . This antibody is tested and proven to work in the following applications: |
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Anti-HIRIP3 antibody |
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| PAab03871 | Lifescience Market | 100 ug | EUR 494.4 |
anti- HIRIP3 antibody |
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| FNab03871 | FN Test | 100µg | EUR 702 |
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Description: Antibody raised against HIRIP3 |
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Anti-HIRIP3 antibody |
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| STJ29094 | St John's Laboratory | 100 µl | EUR 332.4 |
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Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
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HIRIP3 siRNA |
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| 20-abx919397 | Abbexa |
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HIRIP3 siRNA |
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| 20-abx919398 | Abbexa |
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anti-HIRIP3 |
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| YF-PA15655 | Abfrontier | 50 ul | EUR 435.6 |
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Description: Mouse polyclonal to HIRIP3 |
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anti-HIRIP3 |
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| YF-PA15656 | Abfrontier | 50 ug | EUR 435.6 |
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Description: Mouse polyclonal to HIRIP3 |
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pOTB7-HIRIP3 |
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| PVT18716 | Lifescience Market | 2 ug | EUR 277.2 |
Polyclonal HIRIP3 Antibody (Center) |
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| APR16639G | Leading Biology | 0.1ml | EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HIRIP3 (Center). This antibody is tested and proven to work in the following applications: |
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HIRIP3 Polyclonal Conjugated Antibody |
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| C30792 | SAB | 100ul | EUR 476.4 |
HIRIP3 Polyclonal Conjugated Antibody |
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| C31854 | SAB | 100ul | EUR 476.4 |
HIRIP3 Blocking Peptide |
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| 33R-8235 | Fitzgerald | 100 ug | EUR 216 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of HIRIP3 antibody, catalog no. 70R-2185 |
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HIRIP3 Rabbit pAb |
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| A7014-100ul | Abclonal | 100 ul | EUR 369.6 |
HIRIP3 Rabbit pAb |
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| A7014-200ul | Abclonal | 200 ul | EUR 550.8 |
HIRIP3 Rabbit pAb |
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| A7014-20ul | Abclonal | 20 ul | EUR 219.6 |
HIRIP3 Rabbit pAb |
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| A7014-50ul | Abclonal | 50 ul | EUR 267.6 |
HIRIP3 cloning plasmid |
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| CSB-CL883612HU-10ug | Cusabio | 10ug | EUR 451.2 |
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Description: A cloning plasmid for the HIRIP3 gene. |
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Anti-HIRIP3 (3B3) |
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| YF-MA11069 | Abfrontier | 100 ug | EUR 435.6 |
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Description: Mouse monoclonal to HIRIP3 |
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Human HIRIP3 shRNA Plasmid |
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| 20-abx955558 | Abbexa |
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Mouse HIRIP3 shRNA Plasmid |
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| 20-abx982088 | Abbexa |
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HIRIP3 ELISA KIT|Human |
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| EF010125 | Lifescience Market | 96 Tests | EUR 826.8 |
HIRIP3 Recombinant Protein (Human) |
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| RP014686 | ABM | 100 ug | Ask for price |
HIRIP3 Recombinant Protein (Rat) |
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| RP204605 | ABM | 100 ug | Ask for price |
HIRIP3 Recombinant Protein (Mouse) |
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| RP141530 | ABM | 100 ug | Ask for price |
HIRA-Interacting Protein 3 (HIRIP3) Antibody |
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| abx037953-100ug | Abbexa | 100 ug | EUR 469.2 |
HIRA-Interacting Protein 3 (HIRIP3) Antibody |
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| 20-abx005315 | Abbexa |
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HIRA-Interacting Protein 3 (HIRIP3) Antibody |
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| 20-abx113016 | Abbexa |
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HIRA-Interacting Protein 3 (HIRIP3) Antibody |
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| abx030421-400ul | Abbexa | 400 ul | EUR 627.6 |
HIRA-Interacting Protein 3 (HIRIP3) Antibody |
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| abx030421-80l | Abbexa | 80 µl | EUR 343.2 |
HIRA-Interacting Protein 3 (HIRIP3) Antibody |
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| abx233871-100ug | Abbexa | 100 ug | EUR 661.2 |
Hirip3 ORF Vector (Rat) (pORF) |
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| ORF068203 | ABM | 1.0 ug DNA | EUR 607.2 |
HIRIP3 ORF Vector (Human) (pORF) |
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| ORF004896 | ABM | 1.0 ug DNA | EUR 114 |
Hirip3 ORF Vector (Mouse) (pORF) |
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| ORF047178 | ABM | 1.0 ug DNA | EUR 607.2 |
Hirip3 sgRNA CRISPR Lentivector set (Rat) |
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| K7156001 | ABM | 3 x 1.0 ug | EUR 406.8 |
HIRIP3 sgRNA CRISPR Lentivector set (Human) |
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| K0953201 | ABM | 3 x 1.0 ug | EUR 406.8 |
Hirip3 sgRNA CRISPR Lentivector set (Mouse) |
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| K4629701 | ABM | 3 x 1.0 ug | EUR 406.8 |
Hirip3 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K7156002 | ABM | 1.0 ug DNA | EUR 184.8 |
Hirip3 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K7156003 | ABM | 1.0 ug DNA | EUR 184.8 |
Hirip3 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K7156004 | ABM | 1.0 ug DNA | EUR 184.8 |
HIRIP3 sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K0953202 | ABM | 1.0 ug DNA | EUR 184.8 |
HIRIP3 sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K0953203 | ABM | 1.0 ug DNA | EUR 184.8 |
HIRIP3 sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K0953204 | ABM | 1.0 ug DNA | EUR 184.8 |
Hirip3 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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| K4629702 | ABM | 1.0 ug DNA | EUR 184.8 |
Hirip3 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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| K4629703 | ABM | 1.0 ug DNA | EUR 184.8 |
Hirip3 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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| K4629704 | ABM | 1.0 ug DNA | EUR 184.8 |
HIRIP3 Protein Vector (Human) (pPB-C-His) |
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| PV019581 | ABM | 500 ng | EUR 394.8 |
HIRIP3 Protein Vector (Human) (pPB-N-His) |
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| PV019582 | ABM | 500 ng | EUR 394.8 |
HIRIP3 Protein Vector (Human) (pPM-C-HA) |
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| PV019583 | ABM | 500 ng | EUR 394.8 |
HIRIP3 Protein Vector (Human) (pPM-C-His) |
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| PV019584 | ABM | 500 ng | EUR 394.8 |
HIRIP3 Protein Vector (Mouse) (pPB-C-His) |
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| PV188710 | ABM | 500 ng | EUR 723.6 |
HIRIP3 Protein Vector (Mouse) (pPB-N-His) |
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| PV188711 | ABM | 500 ng | EUR 723.6 |
HIRIP3 Protein Vector (Mouse) (pPM-C-HA) |
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| PV188712 | ABM | 500 ng | EUR 723.6 |
HIRIP3 Protein Vector (Mouse) (pPM-C-His) |
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| PV188713 | ABM | 500 ng | EUR 723.6 |
HIRIP3 Protein Vector (Rat) (pPB-C-His) |
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| PV272810 | ABM | 500 ng | EUR 723.6 |
HIRIP3 Protein Vector (Rat) (pPB-N-His) |
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| PV272811 | ABM | 500 ng | EUR 723.6 |
HIRIP3 Protein Vector (Rat) (pPM-C-HA) |
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| PV272812 | ABM | 500 ng | EUR 723.6 |
HIRIP3 Protein Vector (Rat) (pPM-C-His) |
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| PV272813 | ABM | 500 ng | EUR 723.6 |
Hirip3 3'UTR GFP Stable Cell Line |
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| TU255784 | ABM | 1.0 ml | Ask for price |
HIRIP3 3'UTR GFP Stable Cell Line |
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| TU059797 | ABM | 1.0 ml | EUR 1672.8 |
Hirip3 3'UTR Luciferase Stable Cell Line |
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| TU205784 | ABM | 1.0 ml | Ask for price |
Hirip3 3'UTR Luciferase Stable Cell Line |
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| TU109519 | ABM | 1.0 ml | Ask for price |
Hirip3 3'UTR GFP Stable Cell Line |
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| TU159519 | ABM | 1.0 ml | Ask for price |
HIRIP3 3'UTR Luciferase Stable Cell Line |
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| TU009797 | ABM | 1.0 ml | EUR 1672.8 |
Human HIRA-Interacting Protein 3 (HIRIP3) ELISA Kit |
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| abx387793-96tests | Abbexa | 96 tests | EUR 1093.2 |
Human HIRA- interacting protein 3, HIRIP3 ELISA KIT |
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| ELI-38603h | Lifescience Market | 96 Tests | EUR 988.8 |
HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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| LV627643 | ABM | 1.0 ug DNA | EUR 818.4 |
HIRIP3 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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| LV627647 | ABM | 1.0 ug DNA | EUR 818.4 |
HIRIP3 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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| LV627648 | ABM | 1.0 ug DNA | EUR 818.4 |
Mouse HIRA- interacting protein 3, Hirip3 ELISA KIT |
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| ELI-14456m | Lifescience Market | 96 Tests | EUR 1038 |
Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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| K7156005 | ABM | 3 x 1.0 ug | EUR 451.2 |
HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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| K0953205 | ABM | 3 x 1.0 ug | EUR 451.2 |
Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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| K4629705 | ABM | 3 x 1.0 ug | EUR 451.2 |
HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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| LV627644 | ABM | 1.0 ug DNA | EUR 818.4 |
HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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| LV627645 | ABM | 1.0 ug DNA | EUR 888 |
HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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| LV627646 | ABM | 1.0 ug DNA | EUR 888 |
Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1) |
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| K7156006 | ABM | 1.0 ug DNA | EUR 200.4 |
Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2) |
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| K7156007 | ABM | 1.0 ug DNA | EUR 200.4 |
Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3) |
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| K7156008 | ABM | 1.0 ug DNA | EUR 200.4 |
HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) |
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| K0953206 | ABM | 1.0 ug DNA | EUR 200.4 |
HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2) |
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| K0953207 | ABM | 1.0 ug DNA | EUR 200.4 |
HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) |
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| K0953208 | ABM | 1.0 ug DNA | EUR 200.4 |
Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1) |
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| K4629706 | ABM | 1.0 ug DNA | EUR 200.4 |
Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2) |
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| K4629707 | ABM | 1.0 ug DNA | EUR 200.4 |
Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3) |
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| K4629708 | ABM | 1.0 ug DNA | EUR 200.4 |
CD11b Antibody Antibody |
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| ABD2911 | Lifescience Market | 100 ug | EUR 525.6 |
ASAP1 antibody Antibody |
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| DF8746 | Affbiotech | 200ul | EUR 420 |
anti- Antibody^Polyclonal antibody control antibody |
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| LSMab09882 | Lifescience Market | 100 ug | EUR 525.6 |
ARHGDIA Antibody / RHOGDI Antibody |
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| F54788-0.08ML | NSJ Bioreagents | 0.08 ml | EUR 165 |
ARHGDIA Antibody / RHOGDI Antibody |
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| F54788-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 379 |
Antibody |
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| A1360-500 | Biovision | Ask for price | |
Anti-Glycolipid Antibody (AGA) Antibody |
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| 20-abx004855 | Abbexa |
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Ly1 Antibody Reactive (LYAR) Antibody |
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| 20-abx008109 | Abbexa |
|
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Ly1 Antibody Reactive (LYAR) Antibody |
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| 20-abx123734 | Abbexa |
|
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Ly1 Antibody Reactive (LYAR) Antibody |
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| 20-abx014333 | Abbexa |
|
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Ly1 Antibody Reactive (LYAR) Antibody |
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| abx033330-400ul | Abbexa | 400 ul | EUR 627.6 |
Ly1 Antibody Reactive (LYAR) Antibody |
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| abx033330-80l | Abbexa | 80 µl | EUR 343.2 |
Anti-Glycolipid Antibody (AGA) Antibody |
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| abx036399-100ug | Abbexa | 100 ug | EUR 469.2 |
Anti-Glycoprotein Antibody (GP) Antibody |
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| 20-abx319900 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody |
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| 20-abx319901 | Abbexa |
|
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Anti-Glycoprotein Antibody (GP) Antibody |
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| 20-abx319905 | Abbexa |
|
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Anti-Glycoprotein Antibody (GP) Antibody |
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| 20-abx319913 | Abbexa |
|
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Anti-Glycolipid Antibody (AGA) Antibody |
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| abx230204-100ug | Abbexa | 100 ug | EUR 577.2 |
Ly1 Antibody Reactive (LYAR) Antibody |
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| 20-abx324434 | Abbexa |
|
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Ly1 Antibody Reactive (LYAR) Antibody |
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| 20-abx311665 | Abbexa |
|
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Ly1 Antibody Reactive (LYAR) Antibody |
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| abx234901-100ug | Abbexa | 100 ug | EUR 661.2 |
Anti-Anti-SEPT6 antibody antibody |
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| STJ11100949 | St John's Laboratory | 100 µl | EUR 332.4 |
|
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. |
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Anti-Anti-SEPT9 Antibody antibody |
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| STJ111369 | St John's Laboratory | 100 µl | EUR 332.4 |
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Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described. |
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Anti-Anti-SEPT11 Antibody antibody |
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| STJ111530 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT4 Antibody antibody |
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| STJ112276 | St John's Laboratory | 100 µl | EUR 332.4 |
|
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer. |
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Anti-Anti-MARCH9 Antibody antibody |
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| STJ112609 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT11 Antibody antibody |
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| STJ113941 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT11 Antibody antibody |
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| STJ114081 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT5 Antibody antibody |
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| STJ114819 | St John's Laboratory | 100 µl | EUR 332.4 |
|
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
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Anti-Anti-MARCH8 Antibody antibody |
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| STJ114828 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT7 Antibody antibody |
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| STJ116214 | St John's Laboratory | 100 µl | EUR 332.4 |
|
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
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Anti-Anti-SEPT8 Antibody antibody |
|||
| STJ117206 | St John's Laboratory | 100 µl | EUR 332.4 |
|
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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Anti-Anti-SEPT12 Antibody antibody |
|||
| STJ117759 | St John's Laboratory | 100 µl | EUR 332.4 |
|
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. |
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Anti-Anti-MARCH6 Antibody antibody |
|||
| STJ118549 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-MARCH6 Antibody antibody |
|||
| STJ118550 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-MARCH7 Antibody antibody |
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| STJ118752 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT3 Antibody antibody |
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| STJ118990 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT2 Antibody antibody |
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| STJ28365 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT7 Antibody antibody |
|||
| STJ28963 | St John's Laboratory | 100 µl | EUR 332.4 |
|
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. |
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Anti-Anti-SEPT2 Antibody antibody |
|||
| STJ25475 | St John's Laboratory | 100 µl | EUR 332.4 |
Anti-Anti-SEPT5 Antibody antibody |
|||
| STJ25477 | St John's Laboratory | 100 µl | EUR 332.4 |
|
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. |
|||
Anti-Anti-SEPT8 Antibody antibody |
|||
| STJ25479 | St John's Laboratory | 100 µl | EUR 332.4 |
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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Anti-Anti-SEPT1 antibody antibody |
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| STJ119580 | St John's Laboratory | 100 µl | EUR 332.4 |
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Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012] |
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CLCN5 Antibody / CIC-5 antibody |
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| RQ6462 | NSJ Bioreagents | 100ug | EUR 419 |
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Description: The CLCN5 gene encodes the chloride channel Cl-/H+ exchanger ClC-5. This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. |
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Cytokeratin 7 antibody-Cytoskeleton Marker Antibody |
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| 48169-100ul | SAB | 100ul | EUR 399.6 |
Cytokeratin 7 antibody-Cytoskeleton Marker Antibody |
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| 48169-50ul | SAB | 50ul | EUR 286.8 |
Anti CD22 Antibody: CD22 Monoclonal Antibody |
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| 065-A-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: anti-CD22 monoclonal antibody |
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Anti CD22 Antibody: CD22 Monoclonal Antibody |
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| 065-A-1000ug | Virogen | 1000 ug | EUR 1539 |
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Description: anti-CD22 monoclonal antibody |
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Antibody Pair to ApoA-V antibody |
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| 10R-1876 | Fitzgerald | 100 ul | EUR 781.2 |
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Description: Mouse monoclonal Antibody Pair to ApoA-V antibody |
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Ly1 Antibody Reactive Homolog (LYAR) Antibody |
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| 20-abx103034 | Abbexa |
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Ly1 Antibody Reactive Homolog (LYAR) Antibody |
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| 20-abx103035 | Abbexa |
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Ly1 Antibody Reactive Homolog (LYAR) Antibody |
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| 20-abx103036 | Abbexa |
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Hepatitis C Virus Antibody (HCV) Antibody |
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| abx023924-1mg | Abbexa | 1 mg | EUR 1446 |
Anti-Glycoprotein Antibody (GP) Antibody (HRP) |
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| 20-abx319902 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (FITC) |
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| 20-abx319903 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (Biotin) |
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| 20-abx319904 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (HRP) |
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| 20-abx319906 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (FITC) |
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| 20-abx319907 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (Biotin) |
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| 20-abx319908 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (HRP) |
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| 20-abx319914 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (FITC) |
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| 20-abx319915 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (Biotin) |
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| 20-abx319916 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (HRP) |
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| 20-abx319929 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (FITC) |
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| 20-abx319930 | Abbexa |
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Anti-Glycoprotein Antibody (GP) Antibody (Biotin) |
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| 20-abx319931 | Abbexa |
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Ly1 Antibody Reactive (LYAR) Antibody (HRP) |
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| 20-abx311666 | Abbexa |
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Ly1 Antibody Reactive (LYAR) Antibody (FITC) |
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| 20-abx311667 | Abbexa |
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Ly1 Antibody Reactive (LYAR) Antibody (Biotin) |
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| 20-abx311668 | Abbexa |
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Anti-Glycoprotein 210 Antibody (gp210) Antibody |
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| abx233571-100ug | Abbexa | 100 ug | EUR 577.2 |
Anti Deoxyribonucleic Acid Antibody (DNA) Antibody |
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| abx411057-50ug | Abbexa | 50 ug | EUR 710.4 |
Monoclonal NGF/proNGF Neutralizing Antibody Antibody |
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| AMM06679G | Leading Biology | 0.05mg | EUR 633.6 |
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Description: A Monoclonal antibody against Human NGF/proNGF Neutralizing. The antibodies are raised in Mouse. This antibody is applicable in E |
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Goat anti- human Antibody^Polyclonal antibody |
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| LSMab09896 | Lifescience Market | 100 ug | EUR 525.6 |
Antibody for Human alpha Tubulin Antibody |
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| SPC-692D | Stressmarq | 0.1mg | EUR 424.8 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is unconjugated. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-A390 | Stressmarq | 0.1mg | EUR 481.2 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 390. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-A488 | Stressmarq | 0.1mg | EUR 480 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 488. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-A565 | Stressmarq | 0.1mg | EUR 480 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 565. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-A594 | Stressmarq | 0.1mg | EUR 480 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 594. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-A633 | Stressmarq | 0.1mg | EUR 480 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 633. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-A655 | Stressmarq | 0.1mg | EUR 480 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 655. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-A680 | Stressmarq | 0.1mg | EUR 480 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 680. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-A700 | Stressmarq | 0.1mg | EUR 480 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to ATTO 700. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-ALP | Stressmarq | 0.1mg | EUR 472.8 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Alkaline Phosphatase. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-APC | Stressmarq | 0.1mg | EUR 478.8 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to APC . |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-APCCY7 | Stressmarq | 0.1mg | EUR 565.2 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to APC/Cy7. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-BI | Stressmarq | 0.1mg | EUR 475.2 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Biotin. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-DY350 | Stressmarq | 0.1mg | EUR 496.8 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Dylight 350. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-DY405 | Stressmarq | 0.1mg | EUR 483.6 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Dylight 405. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-DY488 | Stressmarq | 0.1mg | EUR 471.6 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Dylight 488. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-DY594 | Stressmarq | 0.1mg | EUR 474 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Dylight 594. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-DY633 | Stressmarq | 0.1mg | EUR 468 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Dylight 633. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-FITC | Stressmarq | 0.1mg | EUR 470.4 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to FITC. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-HRP | Stressmarq | 0.1mg | EUR 465.6 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to HRP. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-P594 | Stressmarq | 0.1mg | EUR 488.4 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to PE/ATTO 594. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-PCP | Stressmarq | 0.1mg | EUR 478.8 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to PerCP. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-RPE | Stressmarq | 0.1mg | EUR 476.4 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to RPE . |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692D-STR | Stressmarq | 0.1mg | EUR 477.6 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is conjugated to Streptavidin. |
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Antibody for Human alpha Tubulin Antibody |
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| SPC-692S | Stressmarq | 0.012mg | EUR 78 |
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Description: A polyclonal antibody for alpha Tubulin from Human. The antibody is produced in rabbit after immunization with human synthetic peptide of Human alpha-Tubulin. The Antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000); ICC/IF (1:100). This alpha Tubulin antibody is unconjugated. |
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Anti-Noelin Antibody BIOTIN Antibody BIOTIN |
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| STJ501938 | St John's Laboratory | 100 µg | EUR 703.2 |
Anti-Noelin Antibody FITC Antibody FITC |
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| STJ501939 | St John's Laboratory | 100 µg | EUR 703.2 |
Anti-Noelin Antibody BIOTIN Antibody BIOTIN |
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| STJ501940 | St John's Laboratory | 100 µg | EUR 703.2 |
The SWI/SNF and RSC chromatin remodeling complexes are both recruited to the HIR-dependent histone genes; SWI/SNF is required for their activation in S phase, whereas RSC is implicated in their repression outside of S phase. Even though their presence at the histone genes is dependent on the HIR complex, their specific recruitment has not been well characterized. In this study we focused on characterizing the role played by the histone chaperone Rtt106 in the cell cycle-dependent recruitment of SWI/SNF and RSC complexes to the histone genes.