The replication-independent histone H3-H4 chaperones HIR, ASF1, and RTT106 co-operate to maintain promoter fidelity.

RNA polymerase II initiates from low complexity sequences so cells must reliably distinguish “real” from “cryptic” promoters and maintain fidelity to the former. Further, this must be performed under a range of conditions, including those found within inactive and highly transcribed regions. Here, we used genome-scale screening to identify those factors that regulate the use of a specific cryptic promoter and how this is influenced by the degree of transcription over the element.
We show that promoter fidelity is most reliant on histone gene transactivators (Spt10, Spt21) and H3-H4 chaperones (Asf1, HIR complex) from the replication-independent deposition pathway. Mutations of Rtt106 that abrogate its interactions with H3-H4 or dsDNA permit extensive cryptic transcription comparable with replication-independent deposition factor deletions. We propose that nucleosome shielding is the primary means to maintain promoter fidelity, and histone replacement is most efficiently mediated in yeast cells by a HIR/Asf1/H3-H4/Rtt106 pathway.

Physical association of Arabidopsis hypersensitive induced reaction proteins (HIRs) with the immune receptor RPS2.

Arabidopsis RPS2 is a typical nucleotide-binding leucine-rich repeat resistance protein, which indirectly recognizes the bacterial effector protein AvrRpt2 and thereby activates effector-triggered immunity (ETI). Previously, we identified two hypersensitive induced reaction (AtHIR) proteins, AtHIR1 (At1g09840) and AtHIR2 (At3g01290), as potential RPS2 complex components.
AtHIR proteins contain the stomatin/prohibitin/flotillin/HflK/C domain (also known as the prohibitin domain or band 7 domain). In this study, we confirmed that AtHIR1 and AtHIR2 form complexes with RPS2 in Arabidopsis and Nicotiana benthamiana using a pulldown assay and fluorescence resonance energy transfer (FRET) analysis.
Arabidopsis has four HIR family genes (AtHIR1-4). All AtHIR proteins could form homo- and hetero-oligomers in vivo and were enriched in membrane microdomains of the plasma membrane. The mRNA levels of all except AtHIR4 were significantly induced by microbe-associated molecular patterns, such as the bacterial flagellin fragment flg22.
Athir2-1 and Athir3-1 mutants allowed more growth of Pto DC3000 AvrRpt2, but not Pto DC3000, indicating that these mutations reduce RPS2-mediated ETI but do not affect basal resistance to the virulent strain. Overexpression of AtHIR1 and AtHIR2 reduced growth of Pto DC3000. Taken together, the results show that the AtHIR proteins are physically associated with RPS2, are localized in membrane microdomains, and quantitatively contribute to RPS2-mediated ETI.

Separation-of-function mutation in HPC2, a member of the HIR complex in S. cerevisiae, results in derepression of the histone genes but does not confer cryptic TATA phenotypes.

The HIR complex, which is comprised of the four proteins Hir1, Hir2, Hir3 and Hpc2, was first characterized as a repressor of three of the four histone gene loci in Saccharomyces cerevisiae. Using a bioinformatical approach, previous studies have identified a region of Hpc2 that is conserved in Schizosaccharomyces pombe and humans. Using a similar approach, we identified two additional domains, CDI and CDII, of the Hpc2 protein that are conserved among yeast species related to S. cerevisiae.
 The replication-independent histone H3-H4 chaperones HIR, ASF1, and RTT106 co-operate to maintain promoter fidelity.
We showed that the N terminal CDI domain (spanning amino acids 63-79) is dispensable for HIR complex assembly, but plays an essential role in the repression of the histone genes by recruiting the HIR complex to the HIR-dependent histone gene loci. The second conserved domain, CDII (spanning amino acids 452-480), is required for the stability of the Hpc2 protein itself as well as for the assembly of the HIR complex.
In addition, we report a novel separation-of-function mutation within CDI of Hpc2, which causes derepression of the histone genes but does not confer other reported hir/hpc- phenotypes (such as Spt phenotypes, heterochromatin silencing defects and repression of cryptic promoters). This is the first direct demonstration that a separation-of-function mutation exists within the HIR complex.

The Saccharomyces cerevisiae histone chaperone Rtt106 mediates the cell cycle recruitment of SWI/SNF and RSC to the HIR-dependent histone genes.

In Saccharomyces cerevisiae, three out of the four histone gene pairs (HTA1-HTB1, HHT1-HHF1, and HHT2-HHF2) are regulated by the HIR co-repressor complex. The histone chaperone Rtt106 has recently been shown to be present at these histone gene loci throughout the cell cycle in a HIR- and Asf1-dependent manner and involved in their transcriptional repression.

HIRIP3 Antibody

E305092 100ug/200ul
EUR 275
Description: Available in various conjugation types.

HIRIP3 antibody

70R-17741 50 ul
EUR 289
Description: Rabbit polyclonal HIRIP3 antibody

HIRIP3 antibody

70R-2185 50 ug
EUR 467
Description: Rabbit polyclonal HIRIP3 antibody raised against the middle region of HIRIP3

HIRIP3 Antibody

GWB-MV194E 50ug Ask for price

anti- HIRIP3 antibody

FNab03871 100µg
EUR 702
Description: Antibody raised against HIRIP3

Polyclonal HIRIP3 Antibody

APR02968G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HIRIP3 . This antibody is tested and proven to work in the following applications:

HIRIP3 Polyclonal Antibody

30792 100ul
EUR 439

HIRIP3 Polyclonal Antibody

30792-100ul 100ul
EUR 302.4

HIRIP3 Polyclonal Antibody

30792-50ul 50ul
EUR 224.4

HIRIP3 Polyclonal Antibody

E-AB-18848-120uL 120uL
EUR 240
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-18848-200uL 200uL
EUR 399
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-18848-20uL 20uL
EUR 73
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-18848-60uL 60uL
EUR 143
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-61469-120uL 120uL
EUR 320
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-61469-200uL 200uL
EUR 530
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-61469-60uL 60uL
EUR 200
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-61469-each each Ask for price
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-52761-120uL 120uL
EUR 240
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-52761-200uL 200uL
EUR 399
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-52761-20uL 20uL
EUR 73
Description: Unconjugated

HIRIP3 Polyclonal Antibody

E-AB-52761-60uL 60uL
EUR 143
Description: Unconjugated

OAAN03627-50UL - HIRIP3 Antibody

OAAN03627-50UL 50ul
EUR 149

Polyclonal HIRIP3 Antibody (Center)

APR16639G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HIRIP3 (Center). This antibody is tested and proven to work in the following applications:

OAAN03627-100UL - HIRIP3 Antibody

OAAN03627-100UL 100ul
EUR 249

OAAN03627-200UL - HIRIP3 Antibody

OAAN03627-200UL 200ul
EUR 389

HIRIP3 Rabbit Polyclonal Antibody

E10G32013 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

HIRIP3 Rabbit Polyclonal Antibody

E10G09929 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

HIRIP3 Rabbit Polyclonal Antibody

E10G02926 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

HIRIP3 Polyclonal Conjugated Antibody

C30792 100ul
EUR 476.4

HIRIP3 Polyclonal Conjugated Antibody

C31854 100ul
EUR 476.4

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx037953-100ug 100 ug
EUR 469.2

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx233871-100ug 100 ug
EUR 661.2

HIRA-Interacting Protein 3 (HIRIP3) Antibody

20-abx113016
  • Ask for price
  • Ask for price
  • 150 ul
  • 50 ul

HIRA-Interacting Protein 3 (HIRIP3) Antibody

20-abx005315
  • Ask for price
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx030421-400ul 400 ul
EUR 627.6

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx030421-80l 80 µl
EUR 343.2

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx005315-100l 100 µl
EUR 400

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx005315-20l 20 µl
EUR 175

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx005315-50l 50 µl
EUR 275

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx037953-100g 100 µg
EUR 337.5

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx113016-100l 100 µl
EUR 612.5

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx233871-100g 100 µg
EUR 350

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx030421-400l 400 µl
EUR 518.75

HIRIP3

CSB-CL883612HU 10 μg plasmid + 200μl Glycerol Ask for price

ARP58286_P050-25UL - HIRIP3 Antibody - middle region

ARP58286_P050-25UL 25ul
EUR 99

ARP58286_P050 - HIRIP3 antibody - middle region (ARP58286_P050)

ARP58286_P050 100ul
EUR 389

HIRIP3 siRNA

20-abx919397
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

HIRIP3 siRNA

20-abx919398
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

HIRIP3 (untagged)-Human HIRA interacting protein 3 (HIRIP3)

SC324592 10 µg Ask for price

HIRIP3 (untagged)-Human HIRA interacting protein 3 (HIRIP3)

SC117881 10 µg Ask for price

Hirip3 (GFP-tagged) - Mouse HIRA interacting protein 3 (Hirip3)

MG209311 10 µg Ask for price

HIRIP3 (GFP-tagged) - Human HIRA interacting protein 3 (HIRIP3)

RG200510 10 µg Ask for price

Hirip3 (untagged) - Mouse HIRA interacting protein 3 (Hirip3), (10ug)

MC219487 10 µg Ask for price

HIRIP3 Rabbit pAb

E2507014 100ul
EUR 225
Description: Available in various conjugation types.

HIRIP3 Rabbit pAb

A7014-100ul 100 ul
EUR 369.6

HIRIP3 Rabbit pAb

A7014-200ul 200 ul
EUR 550.8

HIRIP3 Rabbit pAb

A7014-20ul 20 ul
EUR 219.6

HIRIP3 Rabbit pAb

A7014-50ul 50 ul
EUR 267.6

HIRIP3 (Myc-DDK-tagged)-Human HIRA interacting protein 3 (HIRIP3)

RC200510 10 µg Ask for price

Hirip3 (Myc-DDK-tagged) - Mouse HIRA interacting protein 3 (Hirip3)

MR209311 10 µg Ask for price

HIRIP3 cloning plasmid

CSB-CL883612HU-10ug 10ug
EUR 451.2
Description: A cloning plasmid for the HIRIP3 gene.

Hirip3 (untagged ORF) - Rat HIRA interacting protein 3 (Hirip3), (10 ug)

RN200696 10 µg Ask for price

HIRIP3 Blocking Peptide

33R-8235 100 ug
EUR 119
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of HIRIP3 antibody, catalog no. 70R-2185

HIRIP3 ELISA KIT|Human

EF010125 96 Tests
EUR 826.8

HIRIP3 (Center) Rabbit pAb

E2613959 100ul
EUR 225
Description: Available in various conjugation types.

Human HIRIP3 shRNA Plasmid

20-abx955558
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

Mouse HIRIP3 shRNA Plasmid

20-abx982088
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

Hirip3 (Myc-DDK-tagged ORF) - Rat HIRA interacting protein 3 (Hirip3), (10 ug)

RR200696 10 µg Ask for price

Lenti ORF clone of Hirip3 (mGFP-tagged) - Mouse HIRA interacting protein 3 (Hirip3)

MR209311L4 10 µg Ask for price

HIRIP3 (untagged) - Homo sapiens HIRA interacting protein 3 (HIRIP3), transcript variant 2

SC331200 10 µg Ask for price

HIRIP3 Recombinant Protein (Rat)

RP204605 100 ug Ask for price

Hirip3 ORF Vector (Rat) (pORF)

ORF068203 1.0 ug DNA
EUR 607.2

HIRIP3 Recombinant Protein (Mouse)

RP141530 100 ug Ask for price

HIRIP3 Recombinant Protein (Human)

RP014686 100 ug Ask for price

HIRIP3 (GFP-tagged) - Homo sapiens HIRA interacting protein 3 (HIRIP3), transcript variant 2

RG233362 10 µg Ask for price

HIRIP3 ORF Vector (Human) (pORF)

ORF004896 1.0 ug DNA
EUR 114

Hirip3 ORF Vector (Mouse) (pORF)

ORF047178 1.0 ug DNA
EUR 607.2

Lenti ORF clone of Hirip3 (Myc-DDK-tagged) - Mouse HIRA interacting protein 3 (Hirip3)

MR209311L3 10 µg Ask for price

Human HIRIP3 knockout cell line

ABC-KH6764 1 vial Ask for price
Description: Human HIRIP3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human HIRIP3 knockdown cell line

ABC-KD6764 1 vial Ask for price
Description: Human HIRIP3 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human HIRIP3 Protein Lysate 20ug

IHUHIRIP3PLLY20UG each
EUR 213
Description: Human HIRIP3 Protein Lysate 20ug

HIRIP3 (Myc-DDK tagged) - Homo sapiens HIRA interacting protein 3 (HIRIP3), transcript variant 2

RC233362 10 µg Ask for price

Lenti ORF clone of Hirip3 (mGFP-tagged ORF) - Rat HIRA interacting protein 3 (Hirip3), (10 ug)

RR200696L4 10 µg Ask for price

Lenti ORF particles, Hirip3 (GFP-tagged) - Mouse HIRA interacting protein 3 (Hirip3), 200ul, >10^7 TU/mL

MR209311L4V 200 µl Ask for price

Lenti ORF particles, HIRIP3 (mGFP-tagged) - Human HIRA interacting protein 3 (HIRIP3), 200ul, >10^7 TU/mL

RC200510L4V 200 µl Ask for price

Lenti ORF clone of Hirip3 (Myc-DDK-tagged ORF) - Rat HIRA interacting protein 3 (Hirip3), (10 ug)

RR200696L3 10 µg Ask for price

OCOA13525-20UG - HIRIP3 Protein Lysate

OCOA13525-20UG 20ug
EUR 169

Lenti ORF particles, Hirip3 (GFP-tagged ORF) - Rat HIRA interacting protein 3 (Hirip3), 200ul, >10^7 TU/mL

RR200696L4V 200 µl Ask for price

Lenti ORF particles, HIRIP3 (Myc-DDK tagged) - Human HIRA interacting protein 3 (HIRIP3), 200ul, >10^7 TU/mL

RC200510L3V 200 µl Ask for price

Lenti ORF particles, Hirip3 (Myc-DDK-tagged) - Mouse HIRA interacting protein 3 (Hirip3), 200ul, >10^7 TU/mL

MR209311L3V 200 µl Ask for price

Lenti ORF particles, Hirip3 (Myc-DDK-tagged ORF) - Rat HIRA interacting protein 3 (Hirip3), 200ul, >10^7 TU/mL

RR200696L3V 200 µl Ask for price

HIRIP3 3'UTR GFP Stable Cell Line

TU059797 1.0 ml
EUR 1672.8

Hirip3 3'UTR GFP Stable Cell Line

TU159519 1.0 ml Ask for price

Hirip3 3'UTR GFP Stable Cell Line

TU255784 1.0 ml Ask for price

Hirip3 sgRNA CRISPR Lentivector set (Rat)

K7156001 3 x 1.0 ug
EUR 406.8

HIRIP3 sgRNA CRISPR Lentivector set (Human)

K0953201 3 x 1.0 ug
EUR 406.8

Hirip3 sgRNA CRISPR Lentivector set (Mouse)

K4629701 3 x 1.0 ug
EUR 406.8

HIRIP3 Protein Vector (Rat) (pPM-C-HA)

PV272812 500 ng
EUR 723.6

HIRIP3 Protein Vector (Rat) (pPB-C-His)

PV272810 500 ng
EUR 723.6

HIRIP3 Protein Vector (Rat) (pPB-N-His)

PV272811 500 ng
EUR 723.6

HIRIP3 Protein Vector (Rat) (pPM-C-His)

PV272813 500 ng
EUR 723.6

HIRIP3 Protein Vector (Human) (pPM-C-HA)

PV019583 500 ng
EUR 394.8

HIRIP3 Protein Vector (Mouse) (pPM-C-HA)

PV188712 500 ng
EUR 723.6

AAP58286-100UG - HIRIP3 Peptide - middle region

AAP58286-100UG 100ug
EUR 99

HIRIP3 Protein Vector (Human) (pPB-C-His)

PV019581 500 ng
EUR 394.8

HIRIP3 Protein Vector (Human) (pPB-N-His)

PV019582 500 ng
EUR 394.8

HIRIP3 Protein Vector (Human) (pPM-C-His)

PV019584 500 ng
EUR 394.8

HIRIP3 Protein Vector (Mouse) (pPB-C-His)

PV188710 500 ng
EUR 723.6

HIRIP3 Protein Vector (Mouse) (pPB-N-His)

PV188711 500 ng
EUR 723.6

HIRIP3 Protein Vector (Mouse) (pPM-C-His)

PV188713 500 ng
EUR 723.6

Hirip3 3'UTR Luciferase Stable Cell Line

TU109519 1.0 ml Ask for price

HIRIP3 3'UTR Luciferase Stable Cell Line

TU009797 1.0 ml
EUR 1672.8

Hirip3 3'UTR Luciferase Stable Cell Line

TU205784 1.0 ml Ask for price

Hirip3 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7156002 1.0 ug DNA
EUR 184.8

Hirip3 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7156003 1.0 ug DNA
EUR 184.8

Hirip3 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7156004 1.0 ug DNA
EUR 184.8

HIRIP3 sgRNA CRISPR Lentivector (Human) (Target 1)

K0953202 1.0 ug DNA
EUR 184.8

HIRIP3 sgRNA CRISPR Lentivector (Human) (Target 2)

K0953203 1.0 ug DNA
EUR 184.8

HIRIP3 sgRNA CRISPR Lentivector (Human) (Target 3)

K0953204 1.0 ug DNA
EUR 184.8

Hirip3 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4629702 1.0 ug DNA
EUR 184.8

Hirip3 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4629703 1.0 ug DNA
EUR 184.8

Hirip3 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4629704 1.0 ug DNA
EUR 184.8

Human HIRA- interacting protein 3, HIRIP3 ELISA KIT

ELI-38603h 96 Tests
EUR 988.8

Mouse HIRA- interacting protein 3, Hirip3 ELISA KIT

ELI-14456m 96 Tests
EUR 1038

Human HIRA-Interacting Protein 3 (HIRIP3) ELISA Kit

abx387793-96tests 96 tests
EUR 1093.2

Mouse HIRA-interacting protein 3 (HIRIP3) ELISA Kit

abx527166-96tests 96 tests
EUR 687.5

HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV627643 1.0 ug DNA
EUR 818.4

HIRIP3 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV627647 1.0 ug DNA
EUR 818.4

HIRIP3 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV627648 1.0 ug DNA
EUR 818.4

Hirip3 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each

SR500410 2 nmol Ask for price

Hirip3 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

SR418030 2 nmol Ask for price

HIRIP3 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

SR305556 2 nmol Ask for price

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7156005 3 x 1.0 ug
EUR 451.2

HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0953205 3 x 1.0 ug
EUR 451.2

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4629705 3 x 1.0 ug
EUR 451.2

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K7156006 1.0 ug DNA
EUR 200.4

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K7156007 1.0 ug DNA
EUR 200.4

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K7156008 1.0 ug DNA
EUR 200.4

HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K0953206 1.0 ug DNA
EUR 200.4

HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K0953207 1.0 ug DNA
EUR 200.4

HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K0953208 1.0 ug DNA
EUR 200.4

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4629706 1.0 ug DNA
EUR 200.4

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4629707 1.0 ug DNA
EUR 200.4

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4629708 1.0 ug DNA
EUR 200.4

Hirip3 - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector

TL703037 5 µg/vial Ask for price

HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV627644 1.0 ug DNA
EUR 818.4

Lenti ORF clone of Human HIRA interacting protein 3 (HIRIP3), mGFP tagged

RC200510L4 10 µg Ask for price

Hirip3 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector

TL507050 5 µg/vial Ask for price

HIRIP3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

TL312453 5 µg/vial Ask for price

HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV627645 1.0 ug DNA
EUR 888

HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV627646 1.0 ug DNA
EUR 888

Hirip3 - Rat, 4 unique 29mer shRNA constructs in retroviral untagged vector

TR703037 5 µg/vial Ask for price

Hirip3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

TR507050 5 µg/vial Ask for price

HIRIP3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector

TR312453 5 µg/vial Ask for price

3`UTR clone of HIRA interacting protein 3 (HIRIP3) for miRNA target validation

SC202579 10 µg Ask for price

Lenti ORF clone of Human HIRA interacting protein 3 (HIRIP3), Myc-DDK-tagged

RC200510L3 10 µg Ask for price

Hirip3 - Rat shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

TL703037V 500 ul each Ask for price

Hirip3 - Mouse shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

TL507050V 500 ul each Ask for price

HIRIP3 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

TL312453V 500 ul each Ask for price

Anti-HIRIP3 Antibody

39461 100ul
EUR 479

Anti-HIRIP3 antibody

PAab03871 100 ug
EUR 494.4

Anti-HIRIP3 antibody

STJ29094 100 µl
EUR 332.4
Description: The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Anti-HIRIP3 Antibody

Y058569 100 µl
EUR 145

Rabbit anti-HIRIP3 Antibody

DL98118A-100ul 100 ul
EUR 334
Description: HIRA-interacting protein 3

Rabbit anti-HIRIP3 Antibody

DL98118A-50ul 50 ul
EUR 233.8
Description: HIRA-interacting protein 3

Rabbit anti-HIRIP3 Antibody

YLD3819-100ul 100 ul
EUR 320
Description: Rabbit polyclonal antibody to HIRIP3

Rabbit anti-HIRIP3 Antibody

YLD3819-50ul 50 ul
EUR 200
Description: Rabbit polyclonal antibody to HIRIP3

Rabbit Polyclonal Anti-HIRIP3 Antibody - middle region

TA344388 100 µl Ask for price

anti-HIRIP3

YF-PA15655 50 ul
EUR 435.6
Description: Mouse polyclonal to HIRIP3

anti-HIRIP3

YF-PA15656 50 ug
EUR 435.6
Description: Mouse polyclonal to HIRIP3

Anti-HIRIP3 (3B3)

YF-MA11069 100 ug
EUR 435.6
Description: Mouse monoclonal to HIRIP3

pOTB7-HIRIP3

PVT18716 2 ug
EUR 277.2

HIRA Antibody

43121 100ul
EUR 319

HIRA Antibody

43121-100ul 100ul
EUR 302.4

HIRA Antibody

E043121 100μg/100μl
EUR 255
Description: Available in various conjugation types.

HIRA Antibody

E98461 100ul
EUR 255
Description: Available in various conjugation types.

HIRA Antibody

DF13473 100ul
EUR 420

HIRA Antibody

DF13473-100ul 100ul
EUR 280

HIRA Antibody

DF13473-200ul 200ul
EUR 350

HIRA antibody

70R-35843 100 ug
EUR 294
Description: Rabbit polyclonal HIRA antibody

HIRA Antibody

1-CSB-PA060030
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against HIRA. Recognizes HIRA from Human, Mouse. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/10000

PE Anti-human CD235 Antibody *HIR2*

123511M0 25 tests
EUR 183
Description: HIR2 is an anti-human monoclonal antibody that targets the CD235a antigen.

PE Anti-human CD235 Antibody *HIR2*

123511M0-25tests 25 tests
EUR 179
Description: HIR2 is an anti-human monoclonal antibody that targets the CD235a antigen.

PE Anti-human CD235 Antibody *HIR2*

123511M1 100 tests
EUR 516
Description: HIR2 is an anti-human monoclonal antibody that targets the CD235a antigen.

PE Anti-human CD235 Antibody *HIR2*

123511M1-100tests 100 tests
EUR 505
Description: HIR2 is an anti-human monoclonal antibody that targets the CD235a antigen.

PE Anti-human CD235 Antibody *HIR2*

123511M2 500 tests
EUR 1925
Description: HIR2 is an anti-human monoclonal antibody that targets the CD235a antigen.
The SWI/SNF and RSC chromatin remodeling complexes are both recruited to the HIR-dependent histone genes; SWI/SNF is required for their activation in S phase, whereas RSC is implicated in their repression outside of S phase. Even though their presence at the histone genes is dependent on the HIR complex, their specific recruitment has not been well characterized. In this study we focused on characterizing the role played by the histone chaperone Rtt106 in the cell cycle-dependent recruitment of SWI/SNF and RSC complexes to the histone genes.

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