The replication-independent histone H3-H4 chaperones HIR, ASF1, and RTT106 co-operate to maintain promoter fidelity.

RNA polymerase II initiates from low complexity sequences so cells must reliably distinguish “real” from “cryptic” promoters and maintain fidelity to the former. Further, this must be performed under a range of conditions, including those found within inactive and highly transcribed regions. Here, we used genome-scale screening to identify those factors that regulate the use of a specific cryptic promoter and how this is influenced by the degree of transcription over the element.
We show that promoter fidelity is most reliant on histone gene transactivators (Spt10, Spt21) and H3-H4 chaperones (Asf1, HIR complex) from the replication-independent deposition pathway. Mutations of Rtt106 that abrogate its interactions with H3-H4 or dsDNA permit extensive cryptic transcription comparable with replication-independent deposition factor deletions. We propose that nucleosome shielding is the primary means to maintain promoter fidelity, and histone replacement is most efficiently mediated in yeast cells by a HIR/Asf1/H3-H4/Rtt106 pathway.

Physical association of Arabidopsis hypersensitive induced reaction proteins (HIRs) with the immune receptor RPS2.

Arabidopsis RPS2 is a typical nucleotide-binding leucine-rich repeat resistance protein, which indirectly recognizes the bacterial effector protein AvrRpt2 and thereby activates effector-triggered immunity (ETI). Previously, we identified two hypersensitive induced reaction (AtHIR) proteins, AtHIR1 (At1g09840) and AtHIR2 (At3g01290), as potential RPS2 complex components.
AtHIR proteins contain the stomatin/prohibitin/flotillin/HflK/C domain (also known as the prohibitin domain or band 7 domain). In this study, we confirmed that AtHIR1 and AtHIR2 form complexes with RPS2 in Arabidopsis and Nicotiana benthamiana using a pulldown assay and fluorescence resonance energy transfer (FRET) analysis.
Arabidopsis has four HIR family genes (AtHIR1-4). All AtHIR proteins could form homo- and hetero-oligomers in vivo and were enriched in membrane microdomains of the plasma membrane. The mRNA levels of all except AtHIR4 were significantly induced by microbe-associated molecular patterns, such as the bacterial flagellin fragment flg22.
Athir2-1 and Athir3-1 mutants allowed more growth of Pto DC3000 AvrRpt2, but not Pto DC3000, indicating that these mutations reduce RPS2-mediated ETI but do not affect basal resistance to the virulent strain. Overexpression of AtHIR1 and AtHIR2 reduced growth of Pto DC3000. Taken together, the results show that the AtHIR proteins are physically associated with RPS2, are localized in membrane microdomains, and quantitatively contribute to RPS2-mediated ETI.

Separation-of-function mutation in HPC2, a member of the HIR complex in S. cerevisiae, results in derepression of the histone genes but does not confer cryptic TATA phenotypes.

The HIR complex, which is comprised of the four proteins Hir1, Hir2, Hir3 and Hpc2, was first characterized as a repressor of three of the four histone gene loci in Saccharomyces cerevisiae. Using a bioinformatical approach, previous studies have identified a region of Hpc2 that is conserved in Schizosaccharomyces pombe and humans. Using a similar approach, we identified two additional domains, CDI and CDII, of the Hpc2 protein that are conserved among yeast species related to S. cerevisiae.
 The replication-independent histone H3-H4 chaperones HIR, ASF1, and RTT106 co-operate to maintain promoter fidelity.
We showed that the N terminal CDI domain (spanning amino acids 63-79) is dispensable for HIR complex assembly, but plays an essential role in the repression of the histone genes by recruiting the HIR complex to the HIR-dependent histone gene loci. The second conserved domain, CDII (spanning amino acids 452-480), is required for the stability of the Hpc2 protein itself as well as for the assembly of the HIR complex.
In addition, we report a novel separation-of-function mutation within CDI of Hpc2, which causes derepression of the histone genes but does not confer other reported hir/hpc- phenotypes (such as Spt phenotypes, heterochromatin silencing defects and repression of cryptic promoters). This is the first direct demonstration that a separation-of-function mutation exists within the HIR complex.

The Saccharomyces cerevisiae histone chaperone Rtt106 mediates the cell cycle recruitment of SWI/SNF and RSC to the HIR-dependent histone genes.

In Saccharomyces cerevisiae, three out of the four histone gene pairs (HTA1-HTB1, HHT1-HHF1, and HHT2-HHF2) are regulated by the HIR co-repressor complex. The histone chaperone Rtt106 has recently been shown to be present at these histone gene loci throughout the cell cycle in a HIR- and Asf1-dependent manner and involved in their transcriptional repression.

Polyclonal HIRIP3 Antibody

APR02968G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HIRIP3 . This antibody is tested and proven to work in the following applications:

HIRIP3 Polyclonal Antibody

30792-100ul 100ul
EUR 252

HIRIP3 Polyclonal Antibody

30792-50ul 50ul
EUR 187

anti- HIRIP3 antibody

FNab03871 100µg
EUR 585
Description: Antibody raised against HIRIP3

Anti-HIRIP3 antibody

PAab03871 100 ug
EUR 412

HIRIP3 siRNA

20-abx919397
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

HIRIP3 siRNA

20-abx919398
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

anti-HIRIP3

YF-PA15655 50 ul
EUR 363
Description: Mouse polyclonal to HIRIP3

anti-HIRIP3

YF-PA15656 50 ug
EUR 363
Description: Mouse polyclonal to HIRIP3

pOTB7-HIRIP3

PVT18716 2 ug
EUR 231

Polyclonal HIRIP3 Antibody (Center)

APR16639G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HIRIP3 (Center). This antibody is tested and proven to work in the following applications:

HIRIP3 Polyclonal Conjugated Antibody

C31854 100ul
EUR 397

HIRIP3 Polyclonal Conjugated Antibody

C30792 100ul
EUR 397

HIRIP3 Rabbit pAb

A7014-100ul 100 ul
EUR 308

HIRIP3 Rabbit pAb

A7014-200ul 200 ul
EUR 459

HIRIP3 Rabbit pAb

A7014-20ul 20 ul
EUR 183

HIRIP3 Rabbit pAb

A7014-50ul 50 ul
EUR 223

HIRIP3 cloning plasmid

CSB-CL883612HU-10ug 10ug
EUR 376
Description: A cloning plasmid for the HIRIP3 gene.

Anti-HIRIP3 (3B3)

YF-MA11069 100 ug
EUR 363
Description: Mouse monoclonal to HIRIP3

HIRIP3 Blocking Peptide

33R-8235 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of HIRIP3 antibody, catalog no. 70R-2185

Mouse HIRIP3 shRNA Plasmid

20-abx982088
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human HIRIP3 shRNA Plasmid

20-abx955558
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

HIRIP3 ELISA KIT|Human

EF010125 96 Tests
EUR 689

HIRIP3 Recombinant Protein (Rat)

RP204605 100 ug Ask for price

HIRIP3 Recombinant Protein (Human)

RP014686 100 ug Ask for price

HIRIP3 Recombinant Protein (Mouse)

RP141530 100 ug Ask for price

HIRA-Interacting Protein 3 (HIRIP3) Antibody

20-abx113016
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx037953-100ug 100 ug
EUR 391

HIRA-Interacting Protein 3 (HIRIP3) Antibody

20-abx005315
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx030421-400ul 400 ul
EUR 523

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx030421-80l 80 µl
EUR 286

HIRA-Interacting Protein 3 (HIRIP3) Antibody

abx233871-100ug 100 ug
EUR 551

Hirip3 ORF Vector (Rat) (pORF)

ORF068203 1.0 ug DNA
EUR 506

Hirip3 ORF Vector (Mouse) (pORF)

ORF047178 1.0 ug DNA
EUR 506

HIRIP3 ORF Vector (Human) (pORF)

ORF004896 1.0 ug DNA
EUR 95

HIRIP3 sgRNA CRISPR Lentivector set (Human)

K0953201 3 x 1.0 ug
EUR 339

Hirip3 sgRNA CRISPR Lentivector set (Mouse)

K4629701 3 x 1.0 ug
EUR 339

Hirip3 sgRNA CRISPR Lentivector set (Rat)

K7156001 3 x 1.0 ug
EUR 339

HIRIP3 sgRNA CRISPR Lentivector (Human) (Target 1)

K0953202 1.0 ug DNA
EUR 154

HIRIP3 sgRNA CRISPR Lentivector (Human) (Target 2)

K0953203 1.0 ug DNA
EUR 154

HIRIP3 sgRNA CRISPR Lentivector (Human) (Target 3)

K0953204 1.0 ug DNA
EUR 154

Hirip3 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4629702 1.0 ug DNA
EUR 154

Hirip3 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4629703 1.0 ug DNA
EUR 154

Hirip3 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4629704 1.0 ug DNA
EUR 154

Hirip3 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7156002 1.0 ug DNA
EUR 154

Hirip3 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7156003 1.0 ug DNA
EUR 154

Hirip3 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7156004 1.0 ug DNA
EUR 154

Hirip3 3'UTR GFP Stable Cell Line

TU255784 1.0 ml Ask for price

HIRIP3 3'UTR Luciferase Stable Cell Line

TU009797 1.0 ml
EUR 1394

Hirip3 3'UTR GFP Stable Cell Line

TU159519 1.0 ml Ask for price

Hirip3 3'UTR Luciferase Stable Cell Line

TU205784 1.0 ml Ask for price

HIRIP3 3'UTR GFP Stable Cell Line

TU059797 1.0 ml
EUR 1394

Hirip3 3'UTR Luciferase Stable Cell Line

TU109519 1.0 ml Ask for price

HIRIP3 Protein Vector (Human) (pPB-C-His)

PV019581 500 ng
EUR 329

HIRIP3 Protein Vector (Human) (pPB-N-His)

PV019582 500 ng
EUR 329

HIRIP3 Protein Vector (Human) (pPM-C-HA)

PV019583 500 ng
EUR 329

HIRIP3 Protein Vector (Human) (pPM-C-His)

PV019584 500 ng
EUR 329

HIRIP3 Protein Vector (Rat) (pPB-C-His)

PV272810 500 ng
EUR 603

HIRIP3 Protein Vector (Rat) (pPB-N-His)

PV272811 500 ng
EUR 603

HIRIP3 Protein Vector (Rat) (pPM-C-HA)

PV272812 500 ng
EUR 603

HIRIP3 Protein Vector (Rat) (pPM-C-His)

PV272813 500 ng
EUR 603

HIRIP3 Protein Vector (Mouse) (pPB-C-His)

PV188710 500 ng
EUR 603

HIRIP3 Protein Vector (Mouse) (pPB-N-His)

PV188711 500 ng
EUR 603

HIRIP3 Protein Vector (Mouse) (pPM-C-HA)

PV188712 500 ng
EUR 603

HIRIP3 Protein Vector (Mouse) (pPM-C-His)

PV188713 500 ng
EUR 603

Human HIRA-Interacting Protein 3 (HIRIP3) ELISA Kit

abx387793-96tests 96 tests
EUR 911

Human HIRA- interacting protein 3, HIRIP3 ELISA KIT

ELI-38603h 96 Tests
EUR 824

HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV627643 1.0 ug DNA
EUR 682

HIRIP3 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV627647 1.0 ug DNA
EUR 682

HIRIP3 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV627648 1.0 ug DNA
EUR 682

Mouse HIRA- interacting protein 3, Hirip3 ELISA KIT

ELI-14456m 96 Tests
EUR 865

HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0953205 3 x 1.0 ug
EUR 376

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4629705 3 x 1.0 ug
EUR 376

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7156005 3 x 1.0 ug
EUR 376

HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K0953206 1.0 ug DNA
EUR 167

HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K0953207 1.0 ug DNA
EUR 167

HIRIP3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K0953208 1.0 ug DNA
EUR 167

HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV627644 1.0 ug DNA
EUR 682

HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV627645 1.0 ug DNA
EUR 740

HIRIP3 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV627646 1.0 ug DNA
EUR 740

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4629706 1.0 ug DNA
EUR 167

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4629707 1.0 ug DNA
EUR 167

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4629708 1.0 ug DNA
EUR 167

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K7156006 1.0 ug DNA
EUR 167

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K7156007 1.0 ug DNA
EUR 167

Hirip3 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K7156008 1.0 ug DNA
EUR 167

H2B Antibody Antibody

AF4659 200ul
EUR 376
Description: H2B Antibody Antibody detects endogenous levels of H2B.

CD11b Antibody Antibody

ABD2911 100 ug
EUR 438

anti- Antibody^Polyclonal antibody control antibody

LSMab09882 100 ug
EUR 438

Antibody

A1360-500 Ask for price

Ly1 Antibody Reactive (LYAR) Antibody

20-abx123734
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

Anti-Glycolipid Antibody (AGA) Antibody

abx036399-100ug 100 ug
EUR 391
The SWI/SNF and RSC chromatin remodeling complexes are both recruited to the HIR-dependent histone genes; SWI/SNF is required for their activation in S phase, whereas RSC is implicated in their repression outside of S phase. Even though their presence at the histone genes is dependent on the HIR complex, their specific recruitment has not been well characterized. In this study we focused on characterizing the role played by the histone chaperone Rtt106 in the cell cycle-dependent recruitment of SWI/SNF and RSC complexes to the histone genes.

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